Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Why are two reagents used in the PAXgene Tissue System? FAQ ID - 3603 View Why can I not find the Q-Base device in the device list during the first installation? FAQ ID - 141516 View Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN? FAQ ID -1138 View Why can't I find the DNA methylation qPCR Assay for my gene of interest? FAQ ID -2743 View Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App? FAQ ID - 1414518 View Why did the real-time PCR yield Ct values < 12? FAQ ID -2727 View Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit? FAQ ID -604 View Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035 View Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced? FAQ ID -1844 View Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA? FAQ ID -675 View Why do I get different results for the loci SE33 if I analyze the same sample using the Investigator Nonaplex ESS Kit or Investigator Triplex AFS QS Kit? FAQ ID -2401 View Why do I get genomic DNA contamination in my plasmid prep? FAQ ID -353 View Why do I get smeared PCR products? FAQ ID -87 View Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101 View Why do I have to use an ACS (Assay Control Set)? FAQ ID -2933 View Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 View Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 View Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays? FAQ ID -2713 View Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1? FAQ ID -2575 View Why do I observe a CT shift for the internal control in some samples in comparison to the CT value for the internal control of the dilution series of the Male Control DNA M1? FAQ ID - 3734 View Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1? FAQ ID - 3745 View Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay? FAQ ID -2695 View Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC? FAQ ID -2690 View Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit? FAQ ID -603 View Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits? FAQ ID -1084 View Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase? FAQ ID -2689 View Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon? FAQ ID -2043 View Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate? FAQ ID — 3414 View Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration? FAQ ID -1443 View Why do replicates in real-time PCR have different plateau heights? FAQ ID -539 View Why do the amplification curves generated with ABI PRISM® 7000 SDS instrument look rather zigzag-shaped when compared to results generated with the ABI PRISM® 7700 SDS instrument? FAQ ID -1522 View Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit? FAQ ID -2603 View Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100 View Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 View Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed? FAQ ID -1824 View Why does my DNA sample float out of the slot when loading it onto an agarose gel? FAQ ID -205 View Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97 View Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature? FAQ ID -1083 View Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor? FAQ ID -3132 View Why does my realtime PCR assay quality decrease over time? FAQ ID -589 View Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase? FAQ ID -1053 View Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit? FAQ ID -2390 View Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus? FAQ ID -1999 View Why does the connection test fail? FAQ ID - 141522 View Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System? FAQ ID -1832 View Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column? FAQ ID -3148 View Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 View Why does the fluorescence signal drop sharply at the beginning of an artus® PCR run? FAQ ID -1498 View Why does the miScript Target Protector not induce the degradation process? FAQ ID -2263 View Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on? FAQ ID -1851 View Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? FAQ ID -633 View Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips? FAQ ID -3131 View Why does the QuantiTect Primer Assay for my gene of interest have only one version number? FAQ ID -1134 View Why does the SeqTarget Prep Protocol require a 2-step procedure? FAQ ID -2238 View Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)? FAQ ID -2967 View Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue? FAQ ID -3118 View Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717 View Why have the hazard symbols accompanying products changed? FAQ ID - 3430 View Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer? FAQ ID -1848 View Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays? FAQ ID -2675 View Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 View Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 View Why is a blood collection set required to be used with the PAXgene Blood RNA tube? FAQ ID - 3459 View Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube? FAQ ID - 3628 View Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol. FAQ ID -283 View Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 View Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit? FAQ ID -2068 View Why is DTT used only for semen samples? FAQ ID - 143761 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why is it not recommended to stabilize cells with RNAprotect? FAQ ID -941 View Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 View Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp? FAQ ID -2060 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay? FAQ ID -2686 View Why is my plasmid DNA yield low? FAQ ID -768 View Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping? FAQ ID -2057 View Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits? FAQ ID -1449 View Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced? FAQ ID -1835 View Why is the copy number call lower than expected? FAQ ID — 3413 View Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2987 View Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low? FAQ ID -2574 View Why is the IC not detectable although the analytical PCR is negative? FAQ ID -1506 View Why is the IC not detectable although the analytical PCR is positive? FAQ ID -1505 View Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118 View Why is the initial fluorescence signal too high when using artus PCR assays on the Rotor-Gene cycler? FAQ ID -1497 View Why is the magnesium (Mg2+) solution supplied in a separate tube in the artus® Parvo B19 PCR Kits? FAQ ID -1533 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable? FAQ ID -147395 View Why is the QIAcube Connect App reacting so slowly? FAQ ID - 141519 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers? FAQ ID -1442 View Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits? FAQ ID -1447 View Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits? FAQ ID -1451 View Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits? FAQ ID -1446 View Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs? FAQ ID -2067 View Why is there an asterisk for my miScript Primer Assays? FAQ ID -2176 View Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit? FAQ ID -1620 View Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Why is there no detection of free reporter fluorescence in the LiquiChip System? FAQ ID -732 View Why is there no male DNA obtained from sexual assault samples? FAQ ID - 143767 View Why is there no QuantiTect Primer Assay for my gene of interest? FAQ ID -1140 View Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit? FAQ ID -1993 View Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column? FAQ ID -1044 View Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit? FAQ ID -1755 View Why might my gene of interest drop out during WGA if it is not near a telomere or centromere? FAQ ID -703 View Why should DNA or cDNA targets be less than 250 bp long for real-time PCR? FAQ ID-751 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion? FAQ ID -2412 View Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis? FAQ ID -2188 View Why should I work with diluted or undiluted screens? FAQ ID -2419 View Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation? FAQ ID -862 View Will acetone precipitation recommended in the Qproteome Protocols denature Protein? FAQ ID -807 View Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit? FAQ ID -878 View Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification? FAQ ID -664 View Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843 View Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1199 View Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2991 View Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921 View Will pancreas tissue swim on top of RNAprotect? FAQ ID -536 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand? FAQ ID -1195 View Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue? FAQ ID — 3419 View Will QIAseq FastSelect work in other species? FAQ ID -147911 View Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits? FAQ ID -1553 View Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124 View Will REPLI-g work at high temperatures? FAQ ID -656 View Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121 View Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein? FAQ ID -877 View Will TA cloning work with REPLI-g WGA product? FAQ ID -672 View Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit? FAQ ID -1054 View Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C? FAQ ID - 525 View Will the FastLane Cell cDNA Kit work with suspension cells? FAQ ID -801 View Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively? FAQ ID -1198 View Will the optimized DNA sequence for QIAgenes be provided? FAQ ID -2045 View Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine? FAQ ID -644 View Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823 View Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing? FAQ ID -637 View Will the QIAxcel System run using a green Data Review Software Key? FAQ ID -1853 View Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells? FAQ ID -1202 View Will the random hexamers in the REPLI-g reaction interfere with downstream analysis? FAQ ID -713 View Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA? FAQ ID -1328 View Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library? FAQ ID - 3534 View Will the sequence of the QuantiFast Probe Assays be provided? FAQ ID -2369 View Will the sequences of the QuantiTect Primer Assays be provided? FAQ ID -804 View Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits? FAQ ID -2139 View With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns? FAQ ID -864 View With which platform can I perform my sequencing? FAQ ID - 143072 View With which read length should the EpiTect Hi-C NGS libraries be sequenced FAQ ID - 143073 View Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly? FAQ ID -3136 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care