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Isolation of plasmid DNA from Oligotropha carboxidovorans using the QIAGEN® Plasmid Midi Kit - (EN)
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The procedure has been used successfully for isolation of the large (128 kb), very-low-copynumber (1-2 copies per cell) plasmid pHCG3 and its derivatives from
Oligotropha carboxidovorans
. Yield of plasmid DNA was typically 3-6 µg plasmid DNA from 200 ml culture.
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