Product FAQ

 

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3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
3340 - Which operating system is compatible with PyroMark IdentiFire Software?
3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
3337 - Which method is used for QIAGEN sequencing service?
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
3325 - What are the catalog numbers for the packing materials for the QIAcube?
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?

 

3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323

 

3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
3314-What is the concentration of RNase A sold separately?
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
3302 - How long can I store my purified RNA?
3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
3298-Will the sequence of the QuantiFast Probe Assays be provided?
3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
3292-How do I start using the NeXtal CubicPhase kits?
3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
3272 - Are the QuantiFast Probe Assays wet-lab validated?
3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
3261 - Which purification kits are recommended for the therascreen Pyro kits?
3260 - Which purification kits are recommended for the Pyro kits?
3259 - Which probe labels are available for the QuantiFast Probe Assays?
3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
3253 - What species are QuantiTect Probe Assays offered for?
3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
3248 - What is the difference between sponge, cubic and lamellar phase?
3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
3244 -What is the average length of a QuantiFast Probe Assay?
3243 - What does the Investigator Nonaplex ESS Kit contain?
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
3225 - How pure should my protein be for the NexTal CubicPhase kits?
3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
3217 - How long can I store my purified RNA?
3215 - What is the starting material for SBTexcellerator HLA Kits?
3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
3199 - Which species can be used with the GeneRead rRNA depletion kit?
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
3197 -Where can I find the Population Data for the Investigator HDplex kit?
3196 - What is the DIPsorter Freeware?
3195 - Where can I find the Population Data for the Investigator DIPlex kit?
3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
3192 - Is QIAGEN Protease compatible with Buffer ATL?
3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
3190 - What is the smallest elution volume for RNeasy Mini Spin column?
3188 - How many His residues are required for successful purification on Ni-NTA resins?
3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
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