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Cat. No. / ID. P7720L
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250 U of StableScript Reverse Transcriptase (0.05 mL at 5000 U/mL) and 4x StableScript Reaction Buffer (1 x 1.5 mL)
Cat. No. / ID. P7600L
EnzScript Reverse Transcriptase (10,000 U)
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Cat. No. / ID. P7180L
T7 RNA Polymerase (50,000 U)
50,000 U/mL of T7 RNA Polymerase and 10X T7 RNA Polymerase Buffer
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Cat. No. / ID. RT31-020
Reverse Transcription Kit (20)
20 rxns: 40 µL Enzyme Mix (Reverse Trascriptase and RNase Inhibitor HU), 200 µL 2x RT Master Mix (RT-PCR buffer, oligo(dT)18 primers, random hexamers, MgCl2 and dNTPs mix), 20 µL RNase H (E. coli), 180 µL Nuclease-free water. Store all components at -20°C. The stability of the reagents can be extended if stored at -70°C.
Cat. No. / ID. 205111
Omniscript RT Kit (50)
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Cat. No. / ID. P7460L
Poly(A) Polymerase (1000 U)
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Cat. No. / ID. Y9240L
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Cat. No. / ID. 203123 icon_0368_ls_gen_eco_friendly-s
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Cat. No. / ID. 201203
Taq DNA Polymerase (250 U)
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Cat. No. / ID. 19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
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ssRNA 质量-摩尔换算器
此工具可将 ssRNA 的质量换算为 ssRNA 的摩尔数，以及将 ssRNA 的摩尔数换算为 ssRNA 的质量。
见解
质量-摩尔换算器
ssRNA 摩尔数 [mol] = ssRNA 质量 [g] / RNA 分子量 [g/mol]
ssRNA 末端摩尔数 [mol] = ssRNA 摩尔数 [mol]
ssRNA 拷贝数 [分子] = ssRNA 摩尔数 [mol] x 6.022e23 [分子/mol]
RNA 分子量 [g/mol] = (RNA 长度 [nt] x 321.47 [g/mol//nt]) + 18.02 [g/mol]
提示：321.47 [g/mol]/nt 为未给出序列时的平均值；如果给出序列，则为下列碱基之和，A：329.2 [g/mol]；U：306.2 [g/mol]；C：305.2 [g/mol]；G：345.2 [g/mol]
摩尔-质量换算器
ssRNA 质量 [g] = ssRNA 摩尔数 [mol] x RNA 分子量 [g/mol]
ssRNA 分子量 [g/mol] = (RNA 长度 [nt] x 321.47 [g/mol//nt]) + 18.02 [g/mol]
提示：321.47 [g/mol]/nt 为未给出序列时的平均值；如果给出序列，则为下列碱基之和，A：329.2 [g/mol]；U：306.2 [g/mol]；C：305.2 [g/mol]；G：345.2 [g/mol]
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