推奨製品
Cat. No. / ID. P7720L
StableScript Reverse Transcriptase (250 U)
250 U of StableScript Reverse Transcriptase (0.05 mL at 5000 U/mL) and 4x StableScript Reaction Buffer (1 x 1.5 mL)
Cat. No. / ID. P7600L
EnzScript Reverse Transcriptase (10,000 U)
10,000 U of EnzScript Reverse Transcriptase (0.05 mL at 200,000 U/mL) and 5x M-MLV Reverse Transcriptase RNase H Minus Buffer (1 x 1.5 mL) and 100 mM DTT (1 x 1.5 mL)
Cat. No. / ID. P7180L
T7 RNA Polymerase (50,000 U)
50,000 U/mL of T7 RNA Polymerase and 10X T7 RNA Polymerase Buffer
Cat. No. / ID. L6050L
T4 RNA Ligase 1 (10,000 U)
20,000 U/mL and 10X T4 RNA Ligase Buffer (1 x 1.5 mL)
Cat. No. / ID. RT31-020
Reverse Transcription Kit (20)
20 rxns: 40 µL Enzyme Mix (Reverse Trascriptase and RNase Inhibitor HU), 200 µL 2x RT Master Mix (RT-PCR buffer, oligo(dT)18 primers, random hexamers, MgCl2 and dNTPs mix), 20 µL RNase H (E. coli), 180 µL Nuclease-free water. Store all components at -20°C. The stability of the reagents can be extended if stored at -70°C.
Cat. No. / ID. 205111
Omniscript RT Kit (50)
For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water
Cat. No. / ID. P7460L
Poly(A) Polymerase (1000 U)
1000 U of Poly(A) Polymerase (5000 U/mL) and 10x Poly(A) Polymerase Reaction Buffer.
Cat. No. / ID. Y9240L
RNase Inhibitor (20,000 U)
20,000 U of RNAse Inhibitor (0.5 mL at 40,000 U/mL). Storage temperature: –25°C to –15°C
Cat. No. / ID. 203123 icon_0368_ls_gen_eco_friendly-s
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
Cat. No. / ID. 201203
Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
Cat. No. / ID. 19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
Cat. No. / ID. 203203
HotStarTaq DNA Polymerase (250 U)
250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
Cat. No. / ID. 79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps
一本鎖RNA質量モル数換算ツール
一本鎖RNAの質量をモル数に、モル数を質量に換算します。
洞察
質量からモル数への換算
一本鎖RNAモル数 [mol] = 一本鎖RNA質量 [g] / RNA分子量 [g/mol]
一本鎖RNA末端モル数 [mol] = 一本鎖RNAモル数 [mol]
一本鎖RNAコピー数 [分子数] = 一本鎖RNAモル数 [mol] x 6.022e23 [分子数/mol]
RNA分子量 [g/mol] = (RNA長 [nt] x 321.47 [g/mol//nt]) + 18.02 [g/mol]
注：所定の配列がない場合、または配列を次の合計とする場合の平均分子量は1 ntあたり321.47 [g/mol]とします。A：329.2 [g/mol]；U：306.2 [g/mol]；C：305.2 [g/mol]；G：345.2 [g/mol]
モル数から質量への換算
一本鎖RNA質量 [g] = 一本鎖RNAモル数 [mol] x RNA分子量 [g/mol]
一本鎖RNA分子量 [g/mol] = (RNA長 [nt] x 321.47 [g/mol//nt]) + 18.02 [g/mol]
注：所定の配列がない場合、または配列を次の合計とする場合の平均分子量は1 ntあたり321.47 [g/mol]とします。A：329.2 [g/mol]；U：306.2 [g/mol]；C：305.2 [g/mol]；G：345.2 [g/mol]
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