MagAttract Power Soil Pro DNA Kit

For the hands-free isolation of DNA from soil and stool using your automation workflow of choice

S_1264_2_LS_QF_MagAttractPowerSoilProDNAEP

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MagAttract Power Soil Pro DNA EP Kit

Cat. No. / ID:   47119

For the hands-free isolation of DNA from soil and stool samples on the epMotion® instrument. Requires the separate purchase of the PowerBead Pro Tubes or Plates.
A$7,560.00
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Kit
MagAttract PowerSoilPro DNA EP Kit
MagAttract Power Soil Pro DNA Kit
PowerBead Pro Tubes
PowerBead Pro Plates
The MagAttract Power Soil Pro DNA EP Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • High-quality DNA isolation from tough soil, stool and other environmental samples
  • Improved yield thanks to more efficient PowerBead Pro Plates and Tubes and lysis chemistry
  • Improved inhibitor removal for greater DNA purity
  • Greater downstream accuracy in representation of the microbiome
  • Choose between your preferred automation system: KingFisher Flex or epMotion

Product Details

The MagAttract PowerSoil Pro DNA Kits for the KingFisher and epMotion instruments comprise novel and proprietary methods to isolate microbial genomic DNA from environmental and stool samples. Especially difficult environmental samples, such as compost, sediment and manure, as well as other soil and stool types can be successfully processed with these kits. The kits combine QIAGEN’s second-generation Inhibitor Removal Technology® (IRT) with more efficient bead beating and lysis chemistry. This will result in high-quality DNA that can be immediately used in downstream applications, such as PCR, qPCR and next-generation sequencing (16S and whole-genome).

Performance

The MagAttract PowerSoil Pro DNA Kits are optimized for the Thermo Scientific KingFisher Flex platform and Eppendorf epMotion instrument. They allow the automated high-throughput isolation of DNA from up to 384 samples in less than 1 day.

Principle

The MagAttract PowerSoil Pro DNA Kit is effective at removing PCR inhibitors from soil and stool materials from even the most difficult types. Environmental or human samples are added to a 96-well bead beating plate or bead beating tubes for rapid and thorough homogenization. Cell lysis occurs by a combination of mechanical and chemical methods. Humic acids are removed using IRT. Prepared lysates are transferred to the KingFisher Flex and epMotion platforms where total genomic DNA is captured on specialized magnetic beads in the presence of buffers. DNA is washed on the beads and then eluted, and is ready for NGS, PCR, and other downstream applications.

Both MagAttract PowerSoil Pro DNA Kits have demonstrably improved performances over their respective legacy and competitor kits (See "Better DNA quality with the Pro kits ”). For more information on the performances of both kits, please refer to the Interactive Product Profile.

Procedure

Starting material

The kits are primarily intended to extract microbial genomic DNA from complex substrates, such as soil or stool. Typically, these samples contain high amounts of inhibitors that interfere with downstream enzymatic reactions as well as with compounds that can degrade DNA. The MagAttract PowerSoil Pro DNA Kits are especially designed to remove these contaminants or inhibitors as well as other proteins or nucleases from your samples.

The recommended starting amount of stool samples is 100 mg. The maximum recommended starting material for stool samples is 200 mg; for dehydrated stool samples, start with no more than 100 mg. In general, highest yields are seen with 50–150 mg of input stool material, though this varies with the source. The recommended starting amount of soil material is 250 mg. Microbial genomic DNA purified using the MagAttract PowerSoil Pro DNA Kits is ready for use in enzymatic reactions, such as PCR or NGS, or it can be stored at −30 to −15°C.

Bead beating

Bead beating is required to efficiently lyse the microbial cells of your microbiome sample. The MagAttract PowerSoil Pro DNA Kits are designed to be used in combination with either PowerBead Pro Plates (cat. no. 19311) or PowerBead Pro Tubes (cat. no. 19301). The use of other disruption media can lead to reduced yields of microbial genomic DNA. For convenient high-throughput 96-well homogenization, we offer the TissueLyser II (cat. no. 85300) and Plate Adapter Set (cat. no. 11990). In conjunction with PowerBead Pro Plates, the TissueLyser II provides high-throughput processing for simultaneous, rapid and effective disruption of up to 2 x 96 samples in only a few minutes.

For the disruption using 2 ml PowerBead Pro Tubes, the TissueLyser II provides simultaneous disruption of up to 48 samples in combination with the TissueLyser Adapter Set 2 x 24 (cat. no. 69982) or up to 96 samples in combination with the 2 ml Tube Holder Set (cat. no. 11993) and Plate Adapter Set. 

Homogenization using the Vortex-Genie® 2 with Vortex Adapters for 1.5–2 ml tubes (cat. no. 13000-V1-24) can also be used for the disruption in conjunction with PowerBead Pro Tubes. The order of placement of components and reagents for the platform portion of the protocol will be described in the downloaded software specific to the instrument being used.

Other open platform robots may be used with this kit. However, you may need to contact the manufacturer of your robot for help in adapting this protocol to their system.

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsSoil and stool
Sample size0.25 g of soil or 0.1 g of stool
ProcessingBead beating using PowerBead Pro Tubes or Plates
Storage temperatureSolution CD2 should be stored at 2–8°C upon arrival. All other reagents and kit components of the MagAttract PowerSoil Pro DNA Kit can be stored at room temperature (15–25°C).
Bead sizeMixture of zirconium beads
Throughput96-well plate
For automated processingOn KingFisher and epMotion

Resources

Instrument User Manuals (1)
Brochures & Guides (2)
MagAttract PowerSoil Pro DNA Kit interactive Product Profile
A versatile workflow for the detection of low-abundance microbes
Kit Handbooks (1)
Quick-Start Protocols (1)
Certificates of Analysis (1)
Frequently purchased together
PowerBead Pro Plates (4)
CAT NO./ID 19311
PowerBead Pro Plates (4)
Bead plates ready for rapid and reliable biological sample lysis from a wide variety of starting materials
A$774.00
Plate Adapter Set
CAT NO./ID 11990
Plate Adapter Set
Two sets of adapter plates for use with two 96-well plates on the TissueLyser III & II, compatible with the PowerBead Pro Plates.
A$1,846.00
DNeasy Plant Mini Kit (250)
CAT NO./ID 69106
DNeasy Plant Mini Kit (250)
250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)
A$2,293.00
PowerBead Pro Tubes (2 ml) (50)
CAT NO./ID 19301
PowerBead Pro Tubes (2 ml) (50)
Bead tubes ready for rapid and reliable biological sample lysis from a wide variety of starting materials, 2 ml
A$674.00
S-Blocks (24)
CAT NO./ID 19585
S-Blocks (24)
96-well blocks with 2.2 ml wells, 24 per case
A$966.00
QIAcuity Nanoplate 8.5k 24-well (10)
CAT NO./ID 250011
QIAcuity Nanoplate 8.5k 24-well (10)
10 QIAcuity Nanoplate 8.5k 24-well, 11 Nanoplate Seals
A$591.00
QIAquick PCR Purification Kit (250)
CAT NO./ID 28106
QIAquick PCR Purification Kit (250)
For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
A$974.00
QIAprep 2.0 Spin Miniprep Columns (100 spin columns)
CAT NO./ID 27115
QIAprep 2.0 Spin Miniprep Columns (100 spin columns)
For high-purity plasmid minipreps
A$202.00
HotStarTaq DNA Polymerase (1000 U)
CAT NO./ID 203205
HotStarTaq DNA Polymerase (1000 U)
4 x 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
A$989.00
QIAGEN Plasmid Plus Midi Kit (100)
CAT NO./ID 12945
QIAGEN Plasmid Plus Midi Kit (100)
100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
A$2,250.00
RNase A (17,500 U)
CAT NO./ID 19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution) Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
A$557.00
QIAquick PCR Purification Kit (50)
CAT NO./ID 28104
QIAquick PCR Purification Kit (50)
For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
A$210.00
QIAprep Spin Miniprep Kit (50)
CAT NO./ID 27104
QIAprep Spin Miniprep Kit (50)
For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
A$198.00
RNeasy Mini Kit (250)
CAT NO./ID 74106
RNeasy Mini Kit (250)
250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers.
A$3,225.00
QIAprep Spin Miniprep Kit (250)
CAT NO./ID 27106
QIAprep Spin Miniprep Kit (250)
For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
A$877.00