| Cell Line Species/Tissue: |
human
/
breast adenocarcinoma, epithelial |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
IMEM, 5% FBS |
Percent Serum (%):
|
5 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
|
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
30-40 |
Amount of Nucleic Acid (µg):
|
0.25 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
1 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
20-30% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Passage 100 - 150. Cells from earlier passages tended to clump and did not plate well. |
| References: |
| |