| Cell Line Species/Tissue: |
Human
/
myeloma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEMF12 pen/strep 5% FBS |
Percent Serum (%):
|
5 |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well plate |
Number of Cells:
|
200,000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3 |
Complex Incubation on Cells (hrs):
|
6 |
Analysis Performed Post-Transfection (hrs):
|
24-72 |
Transfection Efficiency (%):
|
>95% |
| Knockdown Efficiency (%): |
>95% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |