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ipsogen Leukemia
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Oncology
ipsogen Leukemia
ipsogen
WT1...
ipsogen
WT1 Profile
Quant
Kit
采用real-time PCR对Wilm瘤(WT1)基因转录本进行定量检测
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
ipsogen
WT1 ProfileQuant Kit (24)
目录编号 / ID.
676913
For 24 reactions: ABL Control Gene Standards, WT1 (exon 1-2) Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix WT1
复制订单详情
需咨询
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
灵敏、准确的定量检测试剂
得到欧洲白血病网络(ELN)联合会专家的优化
即用型解决方案,便利的工作流程
产品详情
ipsogen
WT1 Profile
Quant
Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对Wilm瘤(WT1)基因转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。
绩效
为确保高灵敏度,
ipsogen
WT1 ProfileQuant Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测Wilm瘤(WT1)基因转录本(参见
Accurate detection of Wilm's tumor plasmid standards
和
Reliable detection of ABL plasmid standards
)。
原理
ipsogen
WT1 Profile
Quant
Kit为即用型试剂盒,采用real-time PCR技术,检测Wilm瘤(WT1)基因转录本。该试剂盒能够进行Wilm瘤(WT1)基因转录本在总RNA中相对于ABL对照基因表达的相对定量。该试剂盒灵敏度高,特异性强,可重复性好。
ipsogen
WT1 Profile
Quant
Kit含有特异性的质粒和引物探针混合液,用于定量WT1和ABL基因(参见WT1 gene transcript 和ABL control gene transcript)。该试剂盒包含Wilm瘤(WT1)基因的5个标准品稀释液和ABL基因的3个标准品稀释液使用标准品能够实现准确的定量检测。试剂盒中的试剂在欧洲白血病网络(ELN)联合会专家的共同研究中得以验证。
程序
第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。
ipsogen
WT1 Profile
Quant
Kit可对Wilm瘤(WT1)和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
WT1 Profile
Quant
Kit能够对Wilm瘤(WT1)基因转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。
辅助数据和图表
WT1 gene transcript.
WT1 gene transcript.