Can I multiplex the Microbial DNA qPCR Assays?

Microbial DNA qPCR Assays are a mix of two PCR primers (10 µM each) and one 5′-hydrolysis probe (5 µM) that enables quantitative real-time PCR for 100 reactions. Microbial DNA qPCR Assays are designed using a proprietary and experimentally verified algorithm, providing uniform PCR efficiency and amplification conditions. Each Microbial DNA qPCR Assay undergoes rigorous experimental verification to ensure high PCR efficiency, and this high efficiency is guaranteed when the assays are used with Microbial qPCR Mastermixes and Microbial DNA-Free Water (sold separately). What is the difference between microbial identification and profiling? Identification is determining the microbe’s presence or absence in your sample which requires you to run a No Template Control during your analysis. Profiling is determining the microbe’s relative expression in two or more experimental conditions and you will need to run a reference sample and a normalizer (provided by QIAGEN).

Microbial DNA qPCR Assay Kits are a complete solution for real-time PCR-based microbial detection. Microbial DNA qPCR Assay Kits contain a Microbial DNA qPCR Assay, which is a mix of two PCR primers (10 µM each) and one 5′-hydrolysis probe (5 µM) that enables quantitative real-time PCR for 20 reactions. The kit also contains everything required for a successful qPCR run, including Microbial DNA Positive Control, Positive PCR Control, Microbial DNA-Free Water, and Microbial qPCR Mastermix. What is the difference between microbial identification and profiling? Identification is determining the microbe’s presence or absence in your sample which requires running a No Template Control during your analysis. Profiling is determining the microbe’s relative expression in two or more experimental conditions for which you will need to run a reference sample and a normalizer (provided by QIAGEN).