Transfection Cell Database - Detailed View

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Cell Line: C2C12

Cell Line Species/Tissue:	Mouse / Muscle Myoblast, Fibroblast
Transfection Reagent:	PolyFect
Nucleic Acid:	DNA
Growth Medium:	DMEM
Percent Serum (%):	15
Reporter System:	b-gal
Plasmid Purification Method:
Plate Format:	100-mm dish
Number of Cells:
Percent Confluence(%):
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
Transfection Efficiency (%):

Any modifications to the protocol?:

Notes:
C2C12 cells were transiently co-transfected. Cells were incubated overnight and extracts were prepared 24 h later, normalized by assaying ß-galactosidase activity, immunoprecipitated using anti-CBP antibodies, and analyzed by Western blotting.
References:
Interaction between Acetylated MyoD and the Bromodomain of CBP and/or p300; Anna Polesskaya et al., Molecular and Cellular Biology, Aug. 2001, p. 5312-5320