digital PCR, dPCR, Nanoplate principle

More precision.
More insights.
More time.
Unlock more with digital PCR.

So, you’ve successfully extracted nucleic acids from your precious samples—nice work! Wouldn’t it be great to carry out your most sensitive analyses confidently? With nanoplate digital PCR, you can.

Digital PCR (dPCR) is a method for absolute nucleic acid quantification, combining limiting dilution, end-point PCR and Poisson statistics to partition samples into thousands of reactions for precise measurement.

Nanoplate dPCR enhances this by using microfluidic plates to divide samples into nanoliter-sized wells, each serving as an independent PCR amplification chamber.

It only takes one click to find out more.

Better, right? You’ll be surprised how easy it is to transfer and optimize your current qPCR assays to nanoplate dPCR.

Each dPCR method has strengths suited to specific applications, for example, high accuracy and sensitivity, established method and low-cost potential, but often involve trade-offs in workflow simplicity, time-to-result and throughput. Nanoplate dPCR offers the best balance, but we'll let you decide.
Feature  Nanoplate dPCR  Other dPCR methods (ddPCR, crystal dPCR, chip-based dPCR)
Partition uniformity  High (fixed partitions)  Good (gel-dependent) to variable (droplet size/coalescence)
Contamination risk  Low (sealed partitions)  Moderate (oil-water droplets) to low (gel matrix)
Workflow simplicity  High (qPCR-like)

Moderate (droplet generation) to low (gel preparation)

Automation compatibility  Yes (liquid handlers for plate setup)  Limited to no  
Speed of readout  Fast (simultaneous reading)  Moderate to slower (serial droplet reading)
Multiplexing capability  High  Moderate to limited (channel-, software- and master mix-dependent)
Training requirements  Minimal  High (crystal dPCR) to moderate (ddPCR)

The nanoplate-based QIAcuity Digital PCR System automates all the necessary steps for dPCR - partitioning, thermal cycling, imaging or data acquisition - in a single instrument with flexible configurations for every space and budget. It offers the best balance of versatility, scalability and efficiency.

The technology fuels applications previously hindered by the limitations of qPCR and other dPCR technologies, especially those that require ease of integration into existing workflows with minimum operational complexity.

How fellow researchers benefitted from having a QIAcuity

We’re glad you feel inspired! Whether you’re new to digital PCR, considering a switch, or ready to scale up, our experts are here to help. Need us to help convince your boss? Or are you the boss and still need a little more convincing? Either way, we’re ready when you are.

If you’ve read this far and enjoyed what you learned but aren’t quite ready to take the next step, we understand. Before you go, feel free to share this with your fellow scientists—whether they’re next door or across the globe—and follow our channels for updates. Thank you!

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