
So, you’ve successfully extracted nucleic acids from your precious samples—nice work! Wouldn’t it be great to carry out your most sensitive analyses confidently? With nanoplate digital PCR, you can.
What is it?
Why is it better than qPCR?
Better, right? You’ll be surprised how easy it is to transfer and optimize your current qPCR assays to nanoplate dPCR.
Why is it better than other dPCR methods?
| Feature | Nanoplate dPCR | Other dPCR methods (ddPCR, crystal dPCR, chip-based dPCR) |
| Partition uniformity | High (fixed partitions) | Good (gel-dependent) to variable (droplet size/coalescence) |
| Contamination risk | Low (sealed partitions) | Moderate (oil-water droplets) to low (gel matrix) |
| Workflow simplicity | High (qPCR-like) |
Moderate (droplet generation) to low (gel preparation) |
| Automation compatibility | Yes (liquid handlers for plate setup) | Limited to no |
| Speed of readout | Fast (simultaneous reading) | Moderate to slower (serial droplet reading) |
| Multiplexing capability | High | Moderate to limited (channel-, software- and master mix-dependent) |
| Training requirements | Minimal | High (crystal dPCR) to moderate (ddPCR) |
How is it a fit for my lab?
The nanoplate-based QIAcuity Digital PCR System automates all the necessary steps for dPCR - partitioning, thermal cycling, imaging or data acquisition - in a single instrument with flexible configurations for every space and budget. It offers the best balance of versatility, scalability and efficiency.
The QIAcuity advantage
The technology fuels applications previously hindered by the limitations of qPCR and other dPCR technologies, especially those that require ease of integration into existing workflows with minimum operational complexity.
How fellow researchers benefitted from having a QIAcuity
How can I get to it?
We’re glad you feel inspired! Whether you’re new to digital PCR, considering a switch, or ready to scale up, our experts are here to help. Need us to help convince your boss? Or are you the boss and still need a little more convincing? Either way, we’re ready when you are.