EZ1 RNA Card

For easy setup of RNA purification protocols using the BioRobot EZ1 and EZ1 RNA Kits

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EZ1 RNA Card

Cat. No. / ID: 9015590

Pre-programmed card for EZ1 RNA purification protocols

EZ1 Cards are used in combination with EZ1 kits on the BioRobot EZ1. Please refer to the specific EZ1 kit handbook and the BioRobot EZ1 User Manual for information about product use.

The EZ1 RNA Card is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Easy protocol setup with credit-card ease of use
Standardized processing ensures reproducible results
Increased processing capacity with protocols for cell and tissue samples
Optimized protocols help to ensure reliable purification

Product Details

The EZ1 RNA Card is a preprogrammed card containing protocols for purification of total RNA or total nucleic acids from cells and easy-to-lyse animal and human tissues, and purification of total RNA from any type of human or animal tissue using EZ1 RNA Kits. These protocols provide both on-screen instructions for the user and operating commands for the BioRobot EZ1.

Performance

RNA and nucleic acids purified from cells and tissues with EZ1 RNA kits and the BioRobot EZ1 are of high quality and are suitable for many applications.

Principle

The EZ1 RNA Card is a preprogrammed card containing protocols for purification of total RNA or total nucleic acids from cells and easy-to-lyse animal and human tissues. The EZ1 RNA Card is used in combination with EZ1 RNA kits and the BioRobot EZ1 instrument.

Procedure

Simply insert the EZ1 RNA Card into the BioRobot EZ1 card slot and start the instrument.

Applications

The high-quality RNA obtained using the EZ1 RNA Cell Mini Kit, the EZ1 RNA Tissue Mini Kit, or the EZ1 RNA Universal Tissue Kit and the EZ1 RNA Card is suited for use in many applications, such as:

Gene expression analysis using real-time RT-PCR technologies
Gene expression analysis using microarray technologies
Oncology research

Resources

For purification of total RNA from tissue and cells using EZ1 instruments

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Purification of total RNA from cells using the EZ1 RNA Cell Mini Kit, with and without DNase digest.
Purification of total RNA from standard tissue using the EZ1 RNA Tissue Mini Kit, with and without DNase digest.
Purification of total RNA from any type of tissue using the EZ1 RNA Universal Tissue Kit, with and without DNase digest.

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human cells using the EZ1 RNA Cell Mini Kit'

FAQ ID -974

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human tissues using the EZ1 RNA Tissue Mini Kit'.

FAQ ID -975

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Yes, please follow the User-Developed Protocol 'Purification of total RNA from paraffin-embedded sections using the EZ1 RNA Tissue Mini Kit' (MA33).

FAQ ID -976

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246