Product FAQ - QIAGEN



Product FAQ Bookmark Compartilhar more.. Você não está autorizado a baixar o recurso Organizar opções Sort alphabetically Sort by relevance Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 Visualizar Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 Visualizar Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 Visualizar Can I manually set the threshold line? FAQ ID -2702 Visualizar Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 Visualizar Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 Visualizar Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 Visualizar Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 Visualizar Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 Visualizar At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 Visualizar Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 Visualizar Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 Visualizar Can I transform the Cignal Reporter Assays? FAQ ID -2762 Visualizar Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 Visualizar How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 Visualizar How many housekeeping genes are included in each PCR Array? FAQ ID -2704 Visualizar Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 Visualizar What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 Visualizar How reliable are the results from the miScript Primer Assays? FAQ ID -2730 Visualizar What are EpiTect ChIP qPCR Assays? FAQ ID -2720 Visualizar How do you determine the efficiency using the PCR array? FAQ ID -2701 Visualizar How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 Visualizar How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 Visualizar How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 Visualizar How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 Visualizar How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 Visualizar What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 Visualizar Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 Visualizar What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 Visualizar 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? Visualizar 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? Visualizar 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? Visualizar 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? Visualizar 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? Visualizar 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? Visualizar 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? Visualizar 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? Visualizar What sample types can be tested on the arrays/assays? FAQ ID — 3399 Visualizar What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 Visualizar Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 Visualizar What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 Visualizar Visualizar What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 Visualizar What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 Visualizar 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 Visualizar 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 Visualizar 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 Visualizar 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 Visualizar 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 Visualizar 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 Visualizar 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 Visualizar Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 Visualizar 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? Visualizar 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? Visualizar 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? Visualizar 3314-What is the concentration of RNase A sold separately? Visualizar CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 Visualizar 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? Visualizar Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 Visualizar Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 Visualizar When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 Visualizar Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 Visualizar Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 Visualizar What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 Visualizar Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 Visualizar Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 Visualizar What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 Visualizar Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 Visualizar 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 Visualizar Visualizar 3340 - Which operating system is compatible with PyroMark IdentiFire Software? Visualizar 3325 - What are the catalog numbers for the packing materials for the QIAcube? Visualizar 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? Visualizar 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? Visualizar 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? Visualizar 3337 - Which method is used for QIAGEN sequencing service? Visualizar 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? Visualizar 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? Visualizar 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 Visualizar 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 Visualizar 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? Visualizar 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? Visualizar 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? Visualizar 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? Visualizar 3320 - What is the sensitivity of the Type-it HRM PCR Kit? Visualizar 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? Visualizar 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? Visualizar Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 Visualizar Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 Visualizar 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? Visualizar Visualizar Visualizar Visualizar 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? Visualizar 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? Visualizar 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? Visualizar 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? Visualizar 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? Visualizar What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 Visualizar What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap? FAQ ID - 3364 Visualizar 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? Visualizar 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? Visualizar I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 Visualizar 3302 - How long can I store my purified RNA? Visualizar Do you sell CoralLoad dye separately? FAQ ID - 3368 Visualizar 3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit? Visualizar 3194 - What DNA fragment sizes are required for the Investigator DIPlex kit? Visualizar 3195 - Where can I find the Population Data for the Investigator DIPlex kit? Visualizar 3196 - What is the DIPsorter Freeware? Visualizar 3188 - How many His residues are required for successful purification on Ni-NTA resins? Visualizar 3190 - What is the smallest elution volume for RNeasy Mini Spin column? Visualizar Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 Visualizar What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails -48 Visualizar Is it possible to extract RNA from frozen blood? FAQ ID -3119 Visualizar What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit? FAQ ID -3122 Visualizar Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips? FAQ ID -3131 Visualizar I want to purify RNA from heart tissue. What is the best kit to use? FAQ ID -3116 Visualizar The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why? FAQ ID -3117 Visualizar Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue? FAQ ID -3118 Visualizar Is it possible to partially use RNeasy 96 plates and use the plate multiple times? FAQ ID -3124 Visualizar Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit? FAQ ID -3125 Visualizar Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit? FAQ ID -3100 Visualizar Can I use stained slide materials with IDH 1/2 RGQ PCR kit? FAQ ID -3101 Visualizar Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep spin miniprep kit? FAQ ID -3102 Visualizar How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 Visualizar How many transfections can be performed with Flexiplate siRNAs? FAQ ID -3105 Visualizar What is the standard format for Flexiplate siRNAs delivery? FAQ ID -3104 Visualizar Where to look for ordering Custom siRNAs online? FAQ ID -3106 Visualizar What is the difference between HiPerFect and HiPerFect HTS? FAQ ID -3108 Visualizar Can we do modification XXXXX for custom siRNA that is not on the web page? FAQ ID -3107 Visualizar What is the stability of siRNA lyophilized and resuspended? FAQ ID -3109 Visualizar Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor? FAQ ID -3132 Visualizar What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer? FAQ ID -3111 Visualizar Do you offer FlexiTube or FlexiPlate siRNAs for rat? FAQ ID -3114 Visualizar What is the amplicon size of the PCR product when using the miScript primer assay? FAQ ID -3156 Visualizar What are the stopping points in the QIAamp protocol? FAQ ID -3157 Visualizar What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit? FAQ ID -3167 Visualizar What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24? FAQ ID -3158 Visualizar Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system? FAQ ID -3160 Visualizar Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system? FAQ ID -3161 Visualizar Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument? FAQ ID -3162 Visualizar Do you have data on the DNA yield at different incubation time for the Repli-G Kits? FAQ ID -3113 Visualizar How do I perform the PyroMark Q24 Advanced upgrade? FAQ ID -3163 Visualizar Which operating system is compatible with PyroMark Q24 Advanced application software? FAQ ID -3164 Visualizar What are the differences between new PyroMark Q24 Advanced reagents chemistry compared to PyroMark Q24 Gold reagents? FAQ ID -3165 Visualizar What is the miRNA yield from the miRNeasy Serum/Plasma Kit? FAQ ID -3171 Visualizar What is the adapted annealing protocol for PyroMark Q24 Advanced procedure? FAQ ID -3166 Visualizar What is the RNA size distribution recovered from the miRNeasy Serum/Plasma Kit? FAQ ID -3172 Visualizar What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs? FAQ ID -3174 Visualizar What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry? FAQ ID -3176 Visualizar Does QIAGEN provide template files for GeneMapper® 5 software? FAQ ID -3177 Visualizar Can the Rotor-Gene Q perform gradient PCR? FAQ ID -3183 Visualizar Can Rotor-Gene Reports be modified? FAQ ID -3184 Visualizar What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer? FAQ ID -3179 Visualizar What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q? FAQ ID -3178 Visualizar What to do if there is a loss of communication for the Rotor-Gene Q? FAQ ID -3180 Visualizar Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q? FAQ ID -3181 Visualizar 3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates? Visualizar Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee? FAQ ID - 3186 Visualizar 3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns? Visualizar 3192 - Is QIAGEN Protease compatible with Buffer ATL? Visualizar 3199 - Which species can be used with the GeneRead rRNA depletion kit? Visualizar 3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)? Visualizar What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol? FAQ ID -3173 Visualizar Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly? FAQ ID -3136 Visualizar How do I remove the RNA contamination from purified DNA? FAQ ID -3133 Visualizar How can I know whether there is any genomic DNA present in my purified plasmid? FAQ ID -3134 Visualizar Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat? FAQ ID -3139 Visualizar How do I know whether my miRNA has been successfully purified and how much is expected? FAQ ID -3135 Visualizar What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini? FAQ ID -3140 Visualizar Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight? Visualizar Does RNAprotect affect morphology of tissues? FAQ ID -3142 Visualizar Is RNAprotect also suitable for the stabilization of miRNA? FAQ ID -3143 Visualizar What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit? FAQ ID -3146 Visualizar Are miScript primer assays bench-validated? FAQ ID -3145 Visualizar What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit? FAQ ID -3147 Visualizar Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column? FAQ ID -3148 Visualizar Can single-stranded DNA be removed by gDNA Eliminator columns? FAQ ID -3152 Visualizar How is total RNA purified with RNeasy kits free of genomic DNA contamination? FAQ ID -3154 Visualizar Is it possible to quantify protein content in RLT lysates? FAQ ID -3155 Visualizar Does RNase-free DNase I affect the isolation of dsRNA? FAQ ID -3153 Visualizar What primary cell lines have been tested with HiPerFect Transfection Reagent? FAQ ID -3137 Visualizar Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay? FAQ ID -3138 Visualizar What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY? FAQ ID -3112 Visualizar How long can I store an RLT lysate? FAQ ID -3115 Visualizar What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed? FAQ ID -3175 Visualizar Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood? FAQ ID -3150 Visualizar Is REST software Windows 7 compatible? FAQ ID -3182 Visualizar Is there cross talk between channels in the Rotor-Gene Q? FAQ ID -3185 Visualizar Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit? FAQ ID -3151 Visualizar What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ? FAQ ID -3169 Visualizar What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY? FAQ ID -3168 Visualizar 3197 -Where can I find the Population Data for the Investigator HDplex kit? Visualizar How do I prepare the Spike-in-Control catalog # 219610 to get copies/μl? FAQ ID -3170 Visualizar Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays? FAQ ID — 3416 Visualizar What is the proper disposal procedure for waste from the sample preparation? FAQ ID - 3470 Visualizar Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays? FAQ ID — 3415 Visualizar Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument? FAQ ID - 3479 Visualizar Do you have stability data for RNA isolated with PAXgene miRNA Kit? FAQ ID - 3480 Visualizar What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit? FAQ ID - 3481 Visualizar Can the PAXgene Blood DNA Tubes be stored at 4°C? FAQ ID - 3493 Visualizar How should I thaw frozen PAXgene Blood DNA Tubes? FAQ ID - 3495 Visualizar What is the expected yield from 8.5 ml of blood? FAQ ID - 3496 Visualizar 3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit? FAQ ID - 3497 Visualizar Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long? FAQ ID - 3491 Visualizar Can mitochondrial DNA be isolated with the PAXgene Blood DNA System? FAQ ID - 3498 Visualizar Does the DNA extracted using the PAXgene Blood DNA System contain RNA? FAQ ID - 3499 Visualizar What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays? FAQ ID - 3427 Visualizar Can I multiplex the Microbial DNA qPCR Assays? FAQ ID — 3408 Visualizar Can I measure virulence factor gene expression? FAQ ID — 3405 Visualizar Mostrar mais resultados Back to top Contact QIAGEN Global contacts Technical Service Customer Care