Does the miScript Single Cell qPCR Kit contain controls?
There are control elements for each step contained in the miScript Single Cell qPCR Kit. The miScript SC 3’ Ligation Control (miSC3’) is used to assess 3’ ligation performance. The miScript SC 5’ Ligation Control (miSC5’) is used to assess 5’ ligation performance. The miScript SC Reverse-Transcription Control (miSCRT) is used to assess reverse-transcription performance. The miScript SC PreAMP Control (miSCPA) is used to assess pre-amplification performance. The Positive PCR Control (PPC) is used to assess real-time PCR performance.