How many transfections can be performed with Flexiplate siRNAs?

0.1 nmol scale – add 10 ul of 10uM stock solution.

120 transfections in 96 well plates at 5 nM final concentration OR

60 transfections at 10nM final concentration.

 

0.25 nmol scale - add 25 ul of 10uM stock solution.

300 transfections in 96 well plates at 5 nM final concentration OR 

150 transfections at 10nM final concentration.

 

1.0 nmol scale - add 50 ul of 20uM stock solution.

1200 transfections in 96 well plates at 5 nM final concentration OR 

 600 transfections at 10nM final concentration.