What are the expected DNA yields from tissue processed on the QIAsymphony SP?
DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. For FFPE tissue samples, the DNA yield depends highly on the fixation and processing of the tissue material. QIAsymphony magnetic particles copurify RNA and DNA if both are present in the sample. RNA may influence photometric analysis and may lead to overestimated nucleic acid yields. If RNA-free DNA is required, add RNase A in the step indicated in the respective pretreatment protocol (see protocol sheets for details).