What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit?

Any method for single cell isolation is compatible with the miScript Single Cell qPCR Kit as long as the cells are in 2 µl or less of a neutral buffer like PBS. To avoid any impact on the efficiency of the lysis reaction, carryover of chelators or large amounts of divalent ions should be avoided. We recommend to wash the cell with a neutral buffer such as 1x PBS prior to lysis if your isolation protocol requires such chelators or a high concentration of divalent ions.