Ligation Calculator
This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions.
Insights
Required insert DNA mass [g] = vector DNA mass [g] x {insert DNA length / vector DNA length} x desired insert to vector molar ratio
Notes: Desired ratio is one from set {1, 2, 3, 5 ,7}
Insert length should be smaller than vector length
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