FAQ-2066
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
Yes, special recommendations for detecting underrepresented fragments with the Type-it Mutation Detect PCR Kit can be found in Appendix E of the Type-it Mutation Detect PCR Handbook.
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Type-it Mutation Detect PCR Kit
The Type-it Mutation Detect PCR Kit is especially designed to enable fast and reliable detection of mutations such as deletions, insertions, and translocations. The kit is based on highly specific HotStarTaq Plus DNA Polymerase and a patented buffer system, both of which enable reliable amplification of mutant loci by multiplex PCR without optimization. Subsequent analysis is straightforward and easy and can be carried out on agarose gels, automated electrophoresis instruments, and also by high-resolution capillary sequencing.