FAQ-2002
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
We have data for DNA converted with EpiTect Bisulfite Kits showing that the bisulfite treated DNA is stable for 3 years at -20°C before use in methylation specific PCR with the EpiTect MSP Kit, or the EpiTect MethyLight PCR Kit.
Related products
EpiTect Control DNA and Control DNA Set
EpiTect Control DNAs are ready-to-use, completely methylated or completely unmethylated bisulfite converted DNAs, and untreated, unmethylated genomic DNA, for standardized and reliable control reactions for methylation analysis. The methylated, bisulfite converted DNA as well as unmethylated, bisulfite converted and unconverted DNA are stored in EB Buffer (10 mM Tris Cl) in a ready-to-use solution.
EpiTect Whole Bisulfitome Kit
The EpiTect Whole Bisulfitome Kit provides DNA polymerase, buffers, and reagents for amplification of the entire bisulfite converted genome — the bisulfitome — using multiple displacement amplification technology (MDA). The EpiTect Whole Bisulfitome Kit has been specially developed for the amplification of DNA after conversion with EpiTect Bisulfite Kits. It uses proven REPLI-g technology and has been adapted to the specific requirements of bisulfite converted DNA (smaller DNA fragment size and altered nucleotide composition due to bisulfite conversion), while maintaining the converted sequence representation. Typical DNA yields from the EpiTect Whole Bisulfitome Kit are 1–3 μg per reaction, sufficient for up to 300 PCR analyses from just a single EpiTect bisulfite reaction.
EpiTect MethyLight PCR Kits
The EpiTect MethyLight PCR Kit and the EpiTect MethyLight PCR+ROX Vial Kit enable sensitive and reliable quantification for TaqMan® or dual-labeled probe-based methylation analysis. The kits are available in a ready-to-use format, eliminating the need for further optimization. The kits are compatible with a wide range of real-time cyclers. The EpiTect MethyLight PCR Kit is suitable for most cyclers from Applied Biosystems, with the exception of the Applied Biosystems 7500 systems. The EpiTect MethyLight PCR+ROX Vial Kit is suitable for all other cyclers, including the Applied Biosystems 7500 and instruments from Bio-Rad, Cepheid, Corbett, Eppendorf, Roche, and Stratagene.
EpiTect Bisulfite Kits
EpiTect Bisulfite Kits enable complete conversion of unmethylated cytosines to uracils and subsequent purification in 6 hours. The EpiTect 96 Bisulfite procedure takes less than 7 hours. The highly sensitive method utilizes innovative protection against DNA degradation that guarantees sensitive results, even from 1 ng DNA, and ensures high conversion rates of over 99%. Due to its unique DNA Protect technology, EpiTect Bisulfite converted DNA is highly suitable for whole bisulfitome amplification using EpiTect Whole Bisulfitome Kits. This enables reliable amplification of DNA in cases where bisulfite converted DNA is limited. EpiTect Bisulfite Kits include a spin-column or 96-well format procedure, and can be processed using a centrifuge or vacuum manifold. In addition, the EpiTect Bisulfite Kit can be fully automated on the QIAcube Connect.