| Cell Line Species/Tissue: |
Human
/
umbilical vein endothelial cells |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
EGM |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
56% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Analyzed GFP expression via FACS analysis. Cells were Passage 3, grown on gelatin-coated dishes. |
| References: |
| |