Sample to Insight - QIAGEN
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Transfection Cell Database - Detailed View


Cell Line: 293ft
Cell Line Species/Tissue: Human embryo / Kidney epithelial 
Transfection Reagent: Effectene 
Nucleic Acid:
 DNA 
Growth Medium:
 mem 
Percent Serum (%):
 10 
Reporter System:
 GFP 
Plasmid Purification Method:
 QIAGEN Plasmid Kit 
Plate Format:
 100-mm dish 
Number of Cells:
  
Percent Confluence(%):
 60-70 % 
Amount of Nucleic Acid (µg):
 4 µg 
Amount of Enhancer (µl):
 32 µl 
Amount of Reagent (µl):
 40 µl 
Complex Incubation on Cells (hrs):
 24 h 
Analysis Performed Post-Transfection (hrs):
 48 h 
Transfection Efficiency (%):
 85 % 
 
Any modifications to the protocol?:
 
Notes:
 
References:
 
Sample to Insight - QIAGEN
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