| Cell Line Species/Tissue: |
Human
/
Cervix; epithelial; adenocarcinoma |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10 % |
Reporter System:
|
immunoprecipitation |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
100-mm dish |
Number of Cells:
|
2x10^6 |
Percent Confluence(%):
|
60% |
Amount of Nucleic Acid (µg):
|
10µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
72µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
50-60% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Compared PolyFect Reagent with SuperFect Reagent and recived higher transfection efficiencies using PolyFect Reagent. Achieved 30-40% transfection efficiency when using Superfect. |
| References: |
| |