Rotor-Disc 100 Starter Kit

For use with the Rotor-Gene Q

S_1084_5_GEN_V2
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Rotor-Disc 100 Starter Kit (115V)

Cat no. / ID.   9001602

3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor
Product
Rotor-Disc 100 Starter Kit (115V)
Rotor-Disc 100 Starter Kit (230V)
The Rotor-Disc 100 Starter Kit is for use with the Rotor-Gene Q thermal cycler.
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Explore the virtual tour to learn more and see this product in action.

Features

  • Accelerated reaction setup and high-throughput
  • Outstanding performance in real-time PCR with the Rotor-Gene Q

Product Details

The Rotor-Disc 100 Starter Kit includes all you need for runs of 100 reactions on the Rotor-Gene Q real-time cycler.

Principle

The Rotor-Disc 100 Starter Kit enables easy setup of real-time PCR experiments on the Rotor-Gene Q using the Rotor-Disc 100 format. The kit includes Rotor-Disc 100 packs, a Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, a Rotor-Disc 100 Rotor and Locking Ring, a Rotor-Disc 100 Loading Block, and a Rotor-Disc Pipetting Aid.

Rotor-Discs are fully compatible with the QIAgility instrument, which enables automated reaction setup.

Procedure

The Rotor-Disc 100 Rotor and Rotor-Disc 100 Locking Ring are used with the Rotor-Disc 100. The Rotor-Disc 100 is a circular plate of 100 vertically oriented reaction wells for high-throughput use. The Rotor-Disc 100 is the rotary equivalent of a 96-well plate but with an additional 4 reference wells. It enables integration of the Rotor-Gene Q with 96-well laboratory workflows. The extra wells can be conveniently used for more samples, additional control reactions, or orientation reactions, without occupying any of the standard 96-well positions. For seamless 96-well workflow compatibility, Rotor-Disc 100 wells use 96-well plate labeling conventions, i.e., A1-A12 through to H1-H12. The additional 4 reference wells are labeled R1-R4.

The Rotor-Disc 100 does not use caps. Instead, Rotor-Disc Heat Sealing Film is applied to the top and heat sealed using a Rotor-Disc Heat Sealer. The film prevents contamination by providing a strong, durable, and tamper-proof seal.

Applications

The Rotor-Gene Q and Rotor-Disc 100 Starter Kit enable real-time PCR and high-resolution melting (HRM) applications, including:

  • Gene expression analysis
  • Pathogen detection
  • DNA methylation analysis
  • Genotyping
  • Gene scanning
  • miRNA research

Resources

Brochures and Guides (2)
Now with even more applications!
Rotor-Gene-Q-JA
LITERATURE (1.2MB)
Download
Protocols (1)
Calibration guide for use with QIAgility and the Rotor-Disc 100
User Manuals (1)
Rotor-Disc Heat Sealer
LITERATURE (1.2MB)
Download
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

FAQ

How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.  

 

 

FAQ-1519
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

 

FAQ-2116
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

 

 

FAQ-2117
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

 

FAQ-2119