Ligation Calculator

This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions.

Enter the data of the targets you are interested in and receive sequencing calculations

Required insert DNA mass [g] = vector DNA mass [g] x {insert DNA length / vector DNA length} x desired insert to vector molar ratio

Notes: Desired ratio is one from set {1, 2, 3, 5 ,7}

Insert length should be smaller than vector length

First, the insert and vector DNA is digested using restriction endonucleases to create DNA fragments with sticky (or blunt) ends. Next, the desired DNA fragments are isolated and subsequently joined followed by treatment with DNA ligase. Finally, the resulting intact vector is transformed into competent E. coli cells, and transformants are identified through suitable genetic selection methods.

Want to know more about the step-by-step process of DNA ligation and its typical reaction? Learn more.

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