FAQ-2726
Can I use total RNA for the miRNA PCR Arrays or Assays?
Yes, you can. In fact, total RNA is the recommended starting material for the miScript PCR System. We recommend using the miRNeasy Mini Kit (217004) to isolate total RNA for use with the miScript PCR System.
Related products
miScript miRNA PCR Arrays
miScript miRNA PCR Arrays are mature miRNA-specific forward primers (miScript Primer Assays) that have been arrayed in biologically relevant pathway-focused, disease-focused, or whole miRNome panels. miScript miRNA PCR Arrays are part of the miScript PCR System for miRNA detection and quantification. cDNA is prepared using the miScript II RT Kit with miScript HiSpec Buffer and is used as a template in real-time PCR with a miScript miRNA PCR Array and the miScript SYBR Green PCR Kit. Important note: This product line will be discontinued on May 1, 2021 and will be replaced by the miRCURY LNA miRNA PCR system. We strongly recommend using the following miRCURY LNA PCR products for better miRNA quantification and profiling: miRNome PCR Panels, miRNA Focus PCR Panels and miRNA QC PCR Panel. For more information about this transition, please visit our dedicated knowledge hub or contact us.
Pathway-focused miScript miRNA PCR Arrays
miScript miRNA PCR Arrays are mature miRNA-specific forward primers (miScript Primer Assays) that have been arrayed in biologically relevant pathway-focused, disease-focused or whole miRNome panels. miScript miRNA PCR Arrays are part of the miScript PCR System for miRNA detection and quantification. cDNA is prepared using the miScript II RT Kit with miScript HiSpec Buffer and is used as a template in real-time PCR with a miScript miRNA PCR Array and the miScript SYBR Green PCR Kit. Important note: This product line will be discontinued on May 1, 2021 and will be replaced by the miRCURY LNA miRNA PCR system. We strongly recommend using the following miRCURY LNA PCR products for better miRNA quantification and profiling: miRNome PCR Panels, miRNA Focus PCR Panels and miRNA QC PCR Panel. For more information about this transition, please visit our dedicated knowledge hub or contact us.
miRNeasy Kits
miRNeasy Kits enable purification of total RNA from all types of animal tissues and cells, including difficult-to-lyse tissues and small tissue and cell samples. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required when using the miRNeasy Micro or Mini Kit and an additional RNeasy 96 plate when using the miRNeasy 96 Kit). Purification of 1–12 samples can be automated on the QIAcube Connect. High-throughput processing in convenient 96-well format can be performed using centrifugation or vacuum procedures. The NEW miRNeasy Tissue/Cells Advanced Mini Kit – get excellent results faster without phenol/chloroform using our QIAzol free Advanced chemistry. Try it today.
miRNeasy Tissue/Cells Advanced Kits
The miRNeasy Tissue/Cells Advanced Kits are the second generation of the well-established miRNeasy Kits. These improved versions combine a phenol-free advanced chemistry with gDNA Eliminator Columns or gDNA 96 Eliminator Plates for safe and convenient RNA isolation. They enable purification of total RNA, including small RNAs ≥18 nucleotides, from animal tissues and cells, including small samples. The kits use spin columns for low-throughput RNA purification. The miRNeasy Tissue/Cells Advanced Mini Kit can be automated on the QIAcube Connect.