| Cell Line Species/Tissue: |
rat
/
hepatocytes |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
William`s Medium E without phenol red ( Sigma ) |
Percent Serum (%):
|
5 |
Reporter System:
|
Luciferase, pGL3 (Promega) |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
2 x 10e7 RLU |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| 3 x 10e6 RLU with calcium phosphate |
| References: |
| |