| Cell Line Species/Tissue: |
Mouse
/
embryonic fibroblasts |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
100-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
40-60 |
Amount of Nucleic Acid (µg):
|
15 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
22.5 |
Complex Incubation on Cells (hrs):
|
3.5 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
5-15 |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |