| Cell Line Species/Tissue: |
Human
/
kidney epithelial |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
100-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
70% |
Amount of Nucleic Acid (µg):
|
10µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
10µl |
Complex Incubation on Cells (hrs):
|
24h |
Analysis Performed Post-Transfection (hrs):
|
24h |
Transfection Efficiency (%):
|
80% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Customer compared PolyFect to SuperFect. Both gave 80% transfection efficiency. |
| References: |
| |