QIAcuity Digital PCR System

나노플레이트 기반 디지털 PCR 응용 분야

QIAcuity in action

Explore QIAcuity inside and out while learning more about dPCR.

QIAcuity One, 2plex Device

카탈로그 번호 / ID. 911001

최대 2가지 형광 염료 검출용 1개 플레이트 디지털 PCR 기기, 롤러, USB 플래시 메모리 및 QIAcuity Software Suite: 1회의 예방 유지보수 방문 포함. 또한 공임, 출장 및 부품 1년 보증 포함.

format

One

Four

Eight

multichanneldetection

2 channels (up to 4plex)

8 channels (up to 12plex)

serviceoptions

Device

System

System FUL-2

문의

QIAcuity는 분자생물학 응용 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료 목적으로 사용할 수 없습니다. 따라서 임상 사용을 위한 제품의 성능 특징(즉, 진단, 예후, 치료 또는 혈액은행)은 알려져 있지 않습니다. 디지털 PCR 진단 응용 분야의 경우, QIAcuityDx Digital PCR System을 사용하시기 바랍니다.

QIAcuity in action

Explore QIAcuity inside and out while learning more about dPCR.

Watch tutorials

특징

완전 통합 시스템
확장 가능 형식(1-, 4- 및 8-plate 기기)
고급 멀티플렉싱 기능(최대 12-plex)
최대 6개의 표준 채널과 LSS 염료용 하이브리드 채널 2개
유연성 있는 샘플 처리량
약 2시간 내에 포괄적인 결과 산출

제품 세부 정보

QIAcuity Digital PCR System은 돌연변이 검출, 복제 수 변이(Copy Number Variation, CNV), 유전자 발현 연구, 유전자 편집 분석 등에 대한 정밀하고 멀티플렉싱된 정량화 결과를 제공하도록 설계되었습니다. 이 나노플레이트 기반 시스템은 분획, 열순환(thermocycling) 및 이미징의 표준 dPCR 워크플로우를 최소한의 직접 작업 시간으로 자동화된 플랫폼에 완벽하게 통합합니다.

이 시스템은 QIAcuity Nanoplate, 시약, 분석과 함께 사용됩니다.

가상 데모를 통해 QIAcuity에 대해 자세히 알아보세요.

성능

QIAcuity Digital PCR System은 모든 실험실에서 절대 정량화를 합리적인 비용으로 할 수 있게 합니다. 워크어웨이 자동화는 최소한의 작업 시간으로 분획, 열순환(thermocycling) 및 이미징의 전체 디지털 PCR 워크플로우를 하나의 기기로 통합하여 간소화합니다. 또한 현재 qPCR 분석 항목을 QIAcuity Digital PCR System에 쉽게 적용할 수 있습니다. qPCR에서 전환할 때 플레이트 사용 방식에는 변화가 필요 없으므로 약 2시간 내에 빠른 분석 설정과 빠른 결과를 얻을 수 있습니다.

QIAcuity 기기 - 기능 및 사양

특징	QIAcuity One 2plex	QIAcuity One 5plex	QIAcuity Four	QIAcuity Eight
처리 플레이트	1	1	4	8
검출 채널	2	8¹⁾ (6+2 하이브리드²)	8¹⁾ (6+2 하이브리드²)	8¹⁾ (6+2 하이브리드²)
멀티플렉싱 기능	4	12³⁾¹⁾	12³⁾¹⁾	12³⁾¹⁾
서모사이클러	1	1	1	2
결과까지 걸리는 시간	약 2시간	약 2시간	첫 번째 플레이트는 약 2시간 이후 플레이트당 약 80분	첫 번째 플레이트는 약 2시간 이후 플레이트당 약 40분
처리량(근무일 하루에 처리하는 샘플량)	최대 384개(96-well) 최대 96개(24-well)	최대 384개 (96-well) 최대 96개 (24-well)	최대 672개(96-well) 최대 168개(24-well)	최대 1,248개(96-well) 최대 312개(24-well)

1) 5-plex를 초과하는 멀티플렉싱의 경우 QIAcuity High Multiplex Probe PCR Kit를 사용해야 합니다.
2) 롱 스토크스-시프트(Long Stokes-Shift, LSS) 염료의 경우 하이브리드 채널을 사용합니다.
3) 표준 채널 6개에서 진폭 멀티플렉싱을 사용하여 표적 12개를 동시에 검출할 수 있습니다. 싱글플렉스 LSS 염료와 진폭 멀티플렉싱을 위해 하이브리드 채널을 결합하면 가능한 전체 멀티플렉싱 수가 14개로 늘어납니다. 그러나 이러한 조합은 반응 혼합물에 사용되는 모든 분석에 대한 최적화 요구와 해당 크로스토크 보정으로 인해 사용하지 않는 것이 좋습니다.

원리

피펫팅 및 로딩, 실험 실행, 결과 분석의 간단한 3단계로 약 2시간 내에 원하는 dPCR 결과를 얻을 수 있습니다.

나노플레이트에서의 dPCR 반응 원리는 여기에 설명되어 있습니다.

절차

qPCR 실험과 마찬가지로 샘플 준비에는 마스터 믹스, 프로브 및 프라이머를 96-well 또는 24-well 나노플레이트로 옮긴 다음 샘플을 추가하는 작업이 포함됩니다. 이 시스템은 분획, 열순환, 이미징 기능을 완전 자동화된 하나의 기기에 통합하여 사용자가 2시간 이내에 샘플에서 결과를 얻을 수 있도록 합니다. 사용자는 소프트웨어 제품군에서 분석을 수행할 수 있으며, 표적 염기서열의 마이크로리터당 복제 농도뿐만 아니라 양성 샘플이나 NTC와 같은 정도 관리도 제공됩니다. 이 분석은 또한 동일한 근거리 통신망(Local Area Network, LAN) 내의 원격 컴퓨터로 확장될 수 있습니다.

응용 분야

QIAcuity 기기는 QIAcuity Nanoplate 및 QIAcuity PCR 키트와 함께 다음을 포함한 디지털 PCR 응용 분야를 지원합니다.

희귀 돌연변이 검출
복제 수 변이 분석
유전자 발현 분석
병원체 검출
유전형 분석
miRNA 연구
세포 및 유전자 치료

소프트웨어

기기와 함께 제공되고 별도의 컴퓨터에 설치된 QIAcuity Software Suite는 한 대의 기기에 직접 연결하거나 기존 근거리 통신망(Local Area Network, LAN)을 사용하여 한 대 이상의 QIAcuity 기기를 제어합니다. QIAcuity Software Suite를 사용하여 디지털 PCR 실험, 샘플 및 반응 혼합물을 정의하고 나노플레이트에 배정하고 QIAcuity 기기로 전송할 수 있습니다. 실행 후 데이터를 분석하고 보고서를 만들고 외부 분석을 위해 데이터를 내보낼 수 있습니다. 이 소프트웨어는 반복되는 플레이트 레이아웃 또는 플레이트 실행 매개변수에 쉽게 액세스할 수 있도록 하는 여러 템플릿 기능을 제공하여 디지털 PCR 경험을 더욱 향상시킵니다.

LAN에 통합되면 컴퓨터가 LAN을 통해 클라이언트 역할을 하는 다른 컴퓨터에 액세스할 수 있는 서버로서 QIAcuity Software Suite 기능을 호스팅합니다. 이를 통해 여러 사용자가 여러 컴퓨터에 소프트웨어를 설치하거나 인터넷 연결을 통해 데이터에 액세스하고 교환할 필요 없이 다른 방이나 사무실에서 소프트웨어에 액세스하고 표준 브라우저를 통해 데이터를 분석할 수 있습니다.

서비스

QIAGEN의 다양한 서비스 솔루션으로 기기를 관리하세요. 요구 사항에 맞는 특정 서비스 계약에 대해 알아보세요.

지원되는 데이터 및 수치

간단하고 신속한 플레이트 기반 워크플로우

분획, 서모사이클링 및 이미징이 모두 하나의 완전히 자동화된 기기에 통합되어 2시간 이내에 결과를 제공합니다. 나노플레이트 형식은 샘플 준비를 위한 프런트 엔드 자동화가 가능하여 직접 실험 시간이 더욱 단축됩니다.

QIAcuity Nanoplate dPCR을 사용한 두 번의 독립적 실행에서 반복 가능한 결과로 데이터 정확도가 입증되었습니다.

매우 다양한 존재비의 cDNA 표적의 멀티플렉싱 가능성이 확인되었습니다.

QIAcuity Digital PCR System에서 병렬로 12개의 표적 정량화.

싱글, 듀플렉스 또는 12플렉스 반응을 사용한 QIAcuity 정량화는 매우 일치합니다.

서비스 계획

QIAcuity One 2plex, Full Agreement

카탈로그 번호 / ID. 9245362

1년 동안 출장비, 인건비 및 부품을 포함한 현장 장비 수리 서비스가 제공됩니다. 영업일 기준 2일 안에 대응해 드립니다. Full Agreement 기간 동안 계획된 유지보수 1회 포함.

문의

QIAcuity One, Preventive Subscription

카탈로그 번호 / ID. 9245355

출장비, 인건비 및 유지보수(PM) 부품을 포함한 1회의 현장 예방 정비(Planned Maintenance) 방문이 포함됩니다. 예방 서비스 가입 기간 동안 수리 서비스 10% 할인.

문의

QIAcuity Four, Preventive Subscription

카탈로그 번호 / ID. 9245356

문의

QIAcuity Eight, Preventive Subscription

카탈로그 번호 / ID. 9245357

문의

QIAcuity One 2plex Core Agreement

카탈로그 번호 / ID. 9245358

1년간 출장, 공임 및 예비 부품을 포함한 1회 현장 수리 서비스 및 1회 현장 예방 유지보수. 영업일 기준 5일 대응 시간. 계약 기간 동안 추가 수리 서비스에 대한 10% 할인 포함.

문의

QIAcuity One 5plex Core Agreement

카탈로그 번호 / ID. 9245359

문의

QIAcuity Four Core Agreement

카탈로그 번호 / ID. 9245360

문의

QIAcuity Eight Core Agreement

카탈로그 번호 / ID. 9245361

문의

QIAcuity One 5plex, Full Agreement

카탈로그 번호 / ID. 9245363

문의

QIAcuity Four, Full Agreement

카탈로그 번호 / ID. 9245364

문의

QIAcuity Eight, Full Agreement

카탈로그 번호 / ID. 9245365

문의

QIAcuity One, Installation & Training

카탈로그 번호 / ID. 9245352

본 현장 서비스는 장비 하드웨어 및 시스템 소프트웨어 설치와 최대 4명의 연구원을 대상으로 한 실습 기반 응용 교육을 통해, 실험실 워크플로우에 원활히 통합될 수 있도록 지원합니다.

문의

QIAcuity Four, Installation & Training

카탈로그 번호 / ID. 9245353

문의

QIAcuity Eight, Installation & Training

카탈로그 번호 / ID. 9245354

문의

리소스

For Version 2.0

Version 1.1.3

For Version 2

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.1

Beside of usability improvements and bug fixes, the new features address GMP requirements by offering additional support for 21 CFR part 11 compliance, copy number variation (CNV) analysis, support of future Nanoplates, and improved 1D scatterplot views.

For more information, please refer to the Release Note: QIAcuity Software Suite, Version 2.1

SHA1 checksum: 6D51E273B26FA7D7E0EDA843CB3DCD74B38FA5B1

Version 2.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis

Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Version 1.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.

Note: The installation requires approximately 90 minutes.

Version 2.0

The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:

-Advanced User Management
-Audit Trail
-Signatures

Additional changes compared with the previous software version include the following:

-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.

Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.

Caution: Not following the instructions may result in a loss of your previous plate data!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0

Version 2.1

Beside of improvements and bug fixes, the new features mainly address the GMP requirements by offering additional support for 21 CFR part 11 compliance and get the software ready for future Nanoplate support.

For more information, please refer to the Release Note: QIAcuity Instrument Control Software, Version 2.1.

SHA1 checksum: 0DD41A8DE46D10E81F538ACB71AC184170D39FE0

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

For Version 1.2

Version 2.0

-Advanced User Management
-Audit Trail
-Signatures

Additional changes compared with the previous software version include the following:

SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0

For Version 1.0

The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.

The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.

Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.

Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Fast. Scalable. Reliable.

A versatile workflow for the detection of low-abundance microbes

Updating QIAcuity Software Suite 1.1.3 and Control Software 0.5.2.18 to QIAcuity Software 2.0

Network connection of QIAcuity instruments

For QIAcuity User Manual – Updated QIAcuity Nanoplate Partition Volumes

User manual for QIAcuity instruments and QIAcuity Software 2.1

User manual for QIAcuity instruments and QIAcuity Software 1.2

For use with QIAcuity systems

July 2021

For QIAcuity Software Suite 1.2: Updating VPF for archived or exported plates

User manual for QIAcuity instruments and QIAcuity Software 2.0

FAQ

This is not needed. The QIAcuity is equipped with a flexible power supply technology and operates within a range of 100–240V AC, 50/60 Hz, 1500 VA (max).

FAQ-3761

The instrument software GUI shows error codes including a description and information how to resolve the error. The instrument touchscreen shows an alarm icon in the upper right corner that turns red in case of an instrument failure. Accessing the System Status in the Tool tab allows users to clear errors. Rebooting of the instrument is required to complete the removal of the error. Please do not skip this step. You may always contact QIAGEN Technical Services in case of any question.

FAQ-3763

The user manual contains instructions on how to perform a regular cleaning and decontamination, and how to replace air filters on the QIAcuity instruments. A regular maintenance reduces the dust in the instrument and therefore minimizes the presence of dust particles on the nanoplate, which might interfere with the plate analysis.

FAQ-3765

If you had run a nanoplate for which the installed VPF misses the specific factor, the software will notify you. If you then analyze without the specific VPF, the impact depends on the variation of the partition volume of the new Nanoplate batch compared to the latest. Typically this variation is ±6–7% (approx. 5% CV over the entire plate). The analysis may be repeated after updating the VPF file. After installing the latest VPF and re-analysis of the run, a copy of the plate is generated in the QIAcuity Software Suite including the new results.

FAQ-3769

Yes, the report includes a notification if the matching VPF was missing and, therefore, not applied to the analysis. If the matching VPF was applied there is no notification on the report.

FAQ-3770

If you had analyzed your nanoplates without VFP, the impact depends on the variation of the partition volume of the new nanoplate batch compared to the latest. The VPF reduces the CV from approximately 5% to 2%. We recommend to reanalyze results in case the data originated from different wells (e.g., copy number variations or gene expression data sets for which the reference sample was measured in a different well). Results obtained across different plates should also be r-analyzed. A reanalysis is not required for assay data that were analyzed within the same well (e.g., mutation rate determination using two channels within the same well).

FAQ-3771

A standard PCR plate is required to set up dPCR reaction before transferring it to the nanoplate to ensure a proper mixing of the reaction mix before partitioning.

FAQ-3774

QIAGEN master mixes are optimized for nanoplate microfluidics and are recommended to be used with our dPCR system. They also include an optimized reference dye required for proper analysis.

FAQ-3777

All DNA samples used in reaction mixes should show similar quality and quantity, which can easily be assessed using UV spectrophotometry. DNA samples with an average length of ≥20 kb (e.g., genomic DNA purified via spin column with silica membrane) should be fragmented by restriction digestion before partitioning. Enzymatic fragmentation of larger DNA ensures an even distribution of template throughout the QIAcuity Nanoplate, which in turn leads to an accurate and precise quantification.

FAQ-3778

The QIAcuity Probe PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity Probe PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 12 months, unless otherwise indicated on the label.
The QIAcuity EG PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity EG PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 6 months, unless otherwise indicated on the label.
The QIAcuity Nanoplates does not have expiry date and are stable for at least 1 year when stored at RT.

FAQ-3780

The plate is designed for a single use run. For example, even if only 30 samples are loaded into the 96-well plate, a whole plate will be sealed by the roller. It can't be unsealed and used for another run. The QIAcuity Software won’t allow to set up a separate experiment for the same nanoplate to avoid that previously processed plates being not partitioned a second time.

FAQ-3781

An essential temperature gradient functionality was introduced with software version 2.5. When updating older software versions to 2.5, each QIAcuity instrument will offer the temperature gradient and may be used to find the best cycling temperature for new dPCR assays. When running a QIAcuity Four or a QIAcuity Eight, all plates may have their own temperature profile, including the option for a temperature gradient.

FAQ-3783

The VPF provides a set of well-specific and molding form-specific factors used to specify the exact reaction volume of a nanoplate, thus increasing the concentration calculation of each well.

FAQ-3784

In general, nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. If a new molding form is used for nanoplate manufacturing, potential variation of partition sizes can be addressed by applying the molding form-specific VPF. Thus, each time a new molding form is used, a new VPF is created and made available. Currently, the VPF is updated once every 3–6 months.

FAQ-3785

In dPCR we measure the absolute concentration of targets at endpoint reaction. Concentrations of unknowns can be determined based on dPCR results observed (number of negatives, number of positives, and partition volume analyzed).

FAQ-3786

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