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EZ1&2 DNA Tissue Kit (48)
Cat. No. / ID: 953034
For 48 preps: Reagent Cartridges (Tissue), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer G2, Proteinase K
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The EZ1&2 DNA Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Standardized processing of up to 40 mg tissue samples
- Rapid purification of up to 28 µg high-quality DNA
- Flexible purification from a wide range of tissue types
- Prefilled, sealed reagent cartridges for easy handling
Product Details
The EZ1&2 DNA Tissue Kit contain all required reagents and labware for rapid, automated purification of DNA from up to 40 mg tissue samples using magnetic-particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.
Protocols for automated purification of genomic DNA from a wide range of sample types using the EZ1&2 DNA Tissue Kit are provided in the handbook. These include buccal cells, cultured cells, dried blood and paraffin-embedded tissue sections.
The EZ1&2 DNA Tissue Kit are automated with the EZ2 Connect (1-24 samples per run). These kits can also be used with the EZ1 Advanced XL (1–14 samples per run).
Performance
DNA purified using EZ1&2 DNA Tissue Kit provides high-performance results in downstream applications, such as PCR (see figure “Consistent PCR results”).
See figures
Principle
Automated purification with EZ1&2 DNA Tissue Kit requires use of either EZ2 Connect instruments or EZ1 instruments and the EZ1 Advanced XL DNA Tissue Card.
EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.
Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.
Procedure
Tissues are first incubated in Buffer G2 containing proteinase K until lysed and then homogenized. Pretreated tissues are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1&2 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.
Applications
The high-quality DNA obtained using EZ1&2 DNA Tissue Kit is suited for use in many applications, such as:
- Population genetics and pharmacogenomic research
- PCR and real-time PCR
- Sequencing
- Oncology research
- Genetic identity testing and forensics
- Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies
Specifications
| Features | EZ1&2 DNA Tissue Kit |
|---|---|
| Applications | PCR, real-time RT-PCR, genotyping |
| Elution volume | 50, 100 and 200 µl |
| For automated processing | EZ2 Connect, EZ1 Advanced XL |
| Format | Sample tube |
| Main sample type | Tissue samples |
| Processing | Automated |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | DNA |
| Sample amount | 10–40 mg |
| Technology | Magnetic particles |
| Time per run or per prep | 40 minutes |
| Yield | 9–28 µg |
Supporting data and figures
Consistent PCR results.
DNA was purified from various tissues using the EZ1&2 DNA Tissue procedure. [A] PCR of the bovine obesity single-copy gene (850 bp fragment) using EZ1-purified DNA from bovine tissues. [B] PCR of GAPDH (266 bp fragment) using EZ1-purified DNA from 6 mouse tails. A 2.5 µl aliquot (5%) of each PCR was visualized by agarose gel electrophoresis. M: 100 bp marker; –C: negative control.
Resources
Application Notes (2)
User-Developed Protocols (2)
Kit Handbooks (1)
Brochures & Guides (1)
Quick-Start Protocols (1)
Safety Data Sheets (2)
Certificates of Analysis (1)