EZ1&2 DNA Kits

For automated purification of high-quality DNA from 1–24 samples per run

Features

Rapid purification of up to 9.5 µg DNA from whole blood
Purification of up to 14 µg DNA from buffy coat
Processing of 200–350 µl whole blood samples
Standardized processing of up to 40 mg tissue samples

Product Details

EZ1&2 DNA Kits contain all required reagents and labware for rapid, automated purification of DNA from up to 200 μl blood samples, up to 350 μl blood or buffy coat samples or up to 40 mg tissue samples using magnetic-particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.

EZ1&2 DNA Kits are automated with the EZ2 Connect. These kits can also be used with the EZ1 Advanced XL (1–14 samples per run).

Performance

The automated procedure provides high repeatability (see figures "High-quality DNA from whole blood" and "Reproducible yields of high-quality DNA from whole blood") and linear yields over a range of starting amounts. DNA purified using EZ1&2 DNA Kits gives high-performance results in downstream applications, such as PCR (see figures "High-quality DNA for sensitive and specific analyses" and “Consistent PCR results”).

See figures

High-quality DNA from whole blood.

Reproducible yields of high-quality DNA from whole blood.

High-quality DNA for sensitive and specific analyses.

Consistent PCR results.

Principle

Automated purification with EZ1&2 DNA Kits requires use of either EZ2 Connect instruments or EZ1 instruments and EZ1 DNA Cards (see table “EZ1&2 DNA Kits and EZ1 DNA Cards”).

EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

EZ1&2 DNA Kits and EZ1 DNA Cards

EZ1&2 DNA Kit	EZ1 DNA Card
EZ1&2 DNA Blood 200 µl Kit	EZ1 Advanced XL DNA Blood Card
EZ1&2 DNA Blood 350 µl Kit	EZ1 Advanced XL DNA Blood Card EZ1 Advanced XL DNA Buffy Coat Card
EZ1&2 DNA Tissue Kit	EZ1 Advanced XL DNA Tissue Card

Procedure

Tissues are first incubated in Buffer G2 containing proteinase K until lysed and then homogenized. Whole blood samples (200 µl or 350 µl) and pretreated tissues are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1&2 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.

Applications

The high-quality DNA obtained using EZ1&2 DNA Kits is suited for use in many applications, such as:

Population genetics and pharmacogenomic research
PCR and real-time PCR
Sequencing
Oncology research
Genetic identity testing and forensics
Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies

Comparison of EZ1 DNA Kits

Features	EZ1&2 DNA Blood 200 µl Kit	EZ1&2 DNA Blood 350 µl Kit	EZ1&2 DNA Tissue Kit
Applications	PCR, real-time RT-PCR, genotyping	PCR, real-time RT-PCR, genotyping	PCR, real-time RT-PCR, genotyping
Elution volume	200 µl	200 µl	200 µl
For automated processing	EZ2 Connect, EZ1 Advanced XL	EZ2 Connect, EZ1 Advanced XL	EZ2 Connect, EZ1 Advanced XL
Format	Sample tube	Sample tube	Sample tube
Main sample type	Whole blood	Whole blood	Tissue samples
Processing	Automated	Automated	Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	DNA	DNA	DNA
Sample amount	200 µl	350 µl	10–40 mg
Technology	Magnetic particles	Magnetic particles	Magnetic particles
Time per run or per prep	15–20 minutes	15–20 minutes	40 minutes
Yield	2.6–6 µg	5–12 µg	9–28 µg

Supporting data and figures

High-quality DNA from whole blood.

DNA was purified from 200 µl blood samples using the EZ1&2 DNA Blood 200 µl Kit (upper lanes) and from 350 µl blood samples using the EZ1&2 DNA Blood 350 µl Kit (lower lanes). Lanes 1–6 and 7–12 are from the first and last of 8 processing runs on the EZ1 instrument, respectively. M: 1000 bp DNA ladder (100 ng); +: positive control; -: negative control. 2 µl (1%) of each eluate was visualized on the agarose gel.

Resources

FAQ

Yes, please follow the Supplementary Protocol 'Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA® assays, using EZ1® DNA Blood Kits’.

FAQ ID -1169

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

Yes, please follow the Supplementary Protocol 'Isolation of DNA from cultured cells using the EZ1 DNA Tissue Kit' (MA15).

FAQ ID -990

Making consistent buffy samples takes practice. Serum and erythrocytes are easily isolated alongside the 10x concentrated leukocyte fraction. Our Supplementary Protocol 'Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit' (MA02) contains instructions and recommendations for preparation of buffy coat for DNA isolation on the BioRobot EZ1.

FAQ ID -1249

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

Yes, please check the EZ1 DNA Tissue Handbook for instructions on pretreatment and extraction protocol.

FAQ ID -1260

For storage of DNA purified from forensic samples using the EZ1 DNA Tissue Kit in a buffered solution such as TE rather than water (used for elution in the EZ1 forensic protocols) we suggest the following:

Before storing the DNA, add 1/10 volume of 10x Forensic TE buffer (100 mM Tris·Cl, 1 mM EDTA, pH 8.3) to each sample. For example, add 5 µl of 10x Forensic TE buffer if you eluted in 50 µl. The DNA is ready to use, or can be stored at 2–8°C for 24 h, or at –20°C for longer periods.

FAQ ID -734

Yes, please follow the Supplementary Protocol 'Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit' (MA02).

FAQ ID -985

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246