Products
The QIAamp 96 PowerFecal QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Simple and reliable automated processing for cost and time savings
- No hazardous organic chemicals required
- Efficient removal of PCR inhibitors
- High sensitivity in downstream assays
Product Details
The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of MOBIO's patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.
The QIAamp 96 PowerFecal QIAcube HT Kit is to be discontinued shortly. Try the DNeasy 96 PowerSoil Pro QIAcube HT Kit – for improved performance. Higher yields, better purity, more accurate representation of the microbial diversity.
Performance
QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise quality or reliability.
Effective removal of inhibitors
Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples.Principle
The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
Procedure
The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the MOBIO proprietary Inhibitor Removal Technology (IRT) with two precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations of unbiased DNA.
Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.
Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.
Applications
The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.
Specifications
| Features | Specifications |
|---|---|
| Time per run | 96 samples in approximately 120 minutes 24 samples in approximately 60 minutes |
| Sample amount | Up to 200 mg fresh or frozen stool |
| Throughput | 24-96 samples in increments of 8 samples |
| Elution volume | 100 μl |
Resources
Operating Software (1)
Kit Handbooks (1)
Safety Data Sheets (3)
Quick-Start Protocols (1)
Technical Information (2)
Brochures & Guides (2)
Certificates of Analysis (1)
FAQ
Can I run the QIAcube HT with the QIAxtractor software or vice versa?
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?
What file is required for reporting a QIAcube HT or QIAxtractor issue?
I am getting vacuum errors on my QIAcube HT. What should I do?
1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.
2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.
3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.
Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.
4. Make sure correct plates are used for the selected protocol.
5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.
If the issue persists, please contact QIAGEN Technical Service.
FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?