RT² Profiler PCR Array Modification

• Add up to 4 genes while still retaining patented controls
• A cost-effective way to upgrade your favorite genes to a pathway or disease panel
• Any RT² qPCR Assay can be selected
• Assays laboratory-verified for amplification efficiency, specificity and sensitivity

The RT² Profiler PCR Array Modification combines cataloged RT² Profiler PCR Arrays with up to 4 mRNAs unique to your research, while still retaining the patented controls for reverse transcription efficiency, qPCR performance and genomic DNA contamination. Add your important 4 mRNAs to a cataloged array and make a pathway unique to your research!
To order a RT² Profiler PCR Array Modification, please use this excel file.

Sensitivity

The sensitivity of the RT² First Strand Kit enables gene expression analysis using as little as 1 ng of total RNA. With preamplification, more of your targets can be accurately quantitated using the complete PCR array system.

Reproducibility

The complete PCR array system demonstrates strong correlations across technical replicates, lots and instruments with average correlation coefficients >0.99, ensuring reliable detection of differences in expression between biological samples.

Specificity

The PCR array system, with high-quality input RNA, yields single bands of the predicted size without primer-dimers or other secondary products, therefore providing highly accurate real-time PCR results.

Uniform PCR amplification efficiency

Uniform PCR amplification efficiency is required for the PCR array technology to allow accurate comparisons of gene expression across all genes and all samples. The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees the high performance of every primer assay on PCR arrays.

A guaranteed system

RT² Profiler PCR Arrays are tested and optimized in combination with the RT² SYBR^® Green qPCR Mastermixes and the RT² First Strand Kit. This testing means that RT² Profiler PCR Array performance is guaranteed when all three of these components are used together.

This combination provides the RT² Profiler PCR Array with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory.

RT² Profiler PCR Arrays are sensitive enough for use with RNA prepared from regular samples (0.1–5 µg RNA), FFPE samples and small samples (1–100 ng RNA).

Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate and run the real-time PCR cycling program (see flowchart " Simple procedure"). RT² Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche and Stratagene instruments.

Flexible layout and controls

RT² Profiler PCR Arrays are available in 96-well plate, 384-well plate and 100-well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway, plus 5 housekeeping genes (see " Modified PCR array 96-well plate layout"). Each RT² Profiler PCR Array also includes control elements for:

• Data normalization
• Genomic DNA contamination detection
• RNA sample quality
• General PCR performance

Easy-to-use data analysis

Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔC_T method with normalization of the raw data to either housekeeping genes.

RT² PCR Profiler Arrays can be used in all areas of biological and medical research, including:

• Cancer inflammation and cytokine profiling
• Stem cells
• Neuroscience
• Signal transduction pathways
• Cell adhesion and cell migration
• Biomarker screening and validation