QIAxcel RNA Accessories

For use with QIAxcel capillary gel electrophoresis instruments for automated analysis of RNA samples

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QX RNA Denaturation Buffer (2 x 1.4 ml)

Cat. No. / ID: 929607

2 x 1.4 ml QX RNA Denaturation Buffer

9.700 ¥

Accessories

Buffer

Marker

Plasticware

Instrument Accessories

Buffer type

QX RNA

QX Buffer

For

Denaturation

Dilution

QIAxcel RNA Accessories are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Accessories for automated capillary electrophoresis of RNA samples
For use with QIAxcel RNA Kits and a QIAxcel capillary electrophoresis instrument
Safety, convenience and a streamlined workflow

Product Details

QIAxcel accessories are used in combination with QIAxcel instruments for fully automated sensitive, high-resolution capillary electrophoresis of up to 96 samples per run.
The QIAxcel RNA Accessories only work with the QIAxcel RNA QC Kit v2.0 which is a mandatory component of the QIAxcel Connect System and its predecessor, the QIAxcel Advanced System.

Resources

For calibration of signal intensity

For optimal RNA fragment size determination

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher

PCR 実験における重要項目と新技術

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument

Addressing critical factors and new solutions

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

Download Safety Data Sheets for QIAGEN product components.

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.

FAQ

For each cartridge used with the QIAxcel System, 5 additional intensity calibration runs have been programmed onto the smart key. If i.e. using a QIAxcel DNA High-resolution Cartridge, 105 will be displayed when using the cartridge for the first time – 100 sample runs, plus 5 intensity calibration runs.

FAQ ID -1827

The N2 bottle for the QIAxcel System should be changed when the Press1: in the Instruments Control Panel is “low”.

"Pressure 1" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is sufficient for the current sample run; however, the N2 cylinder should be replaced once the run has finished.

"Pressure 2" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is insufficient for the current sample run and the analysis will not be performed. The N2 cylinder should be replaced.

FAQ ID -1830

Refer to Appendix D of the QIAxcel DNA Handbook for hot water soaking and gel droplet testing. The handbook can be accessed by going to the Resources tab in the link below:

http://www.qiagen.com/products/catalog/automated-solutions/detection-and-analysis/qiaxcel-dna-kits#

FAQ ID -1838

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824

The QIAxcel DNA Fast Analysis Cartridge can only be run on the QIAxcel Fast Analysis System with the BioCalculator Fast Analysis software and the grey software key.

All other QIAxcel Cartridges can only be run on the QIAxcel System with the BioCalculator software and the blue software key.

FAQ ID -1828

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834

The concentration of the DNA marker, pUC18/HaeIII, provided in the QIAxcel DNA Kits is 5ug/ml in 1XTE buffer solution. Customers can run their own DNA size marker sample with different concentration levels using the provided Methods (i.e. M500, H500, L500 and so on).

The recommended method for all basic applications with the QIAxcel System is the M500 method. The BioCalculator software also allows customers to choose their own Sample Injection Time in the Sequence table. The M500 method provides 5KV for 20 seconds for the electro-kinetic injection of lower concentrated DNA samples. If the Injection Time is doubled or tripled (40 or 60 sec), you can expect double or triple intensities (also peak heights).

FAQ ID -1841

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858

Verify that the Cartridge Purge Port label is removed. If it has been removed, verify that the alignment marker and samples are in the appropriate locations in the Buffer Tray and the 96-well Sample Tray used with the QIAxcel System or QIAxcel Advanced?.

FAQ ID -1847

When using this specific marker combination with the QIAxcel System or QIAxcel Advanced, i.e. for STR analysis, the 1.8 kb fragment lies outside of the range of detectable fragments and does not show up on the electropherogram and/or the gel view image.

FAQ ID -1835

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray.
Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day.
Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.

FAQ ID -1823

Data for each cartridge intensity calibration on the QIAxcel System is stored in a single file named 'calibration.log'. This file is saved in the BioCalculator root directory C:\Program Files\BioCalculator.

If, for any reason, a different computer is used than the one containing the calibration.log file, the file should be transferred to the new computer. Otherwise, recalibration of the cartridge is required.

Likewise, if the QIAxcel Gel cartridge is used on a different instrument than the one it was calibrated on, another intensity calibration should be performed.

FAQ ID -1825

If one of the capillaries of the gel cartridge for the QIAxcel System or QIAxcel Advanced is clogged, the corresponding lane in the gel-view image is typically black.

FAQ ID -1836

Check the QIAxcel computer “COM port” in the Windows Control Panel and then select the correct COM port number in the BioCalculator Instrument Control panel. Be sure to turn the Instrument on first prior to launching the BioCalculator software. If there is still “NO COM”, please contact QIAGEN Technical Services.

FAQ ID -1848

High or very low salt concentration could lead to the described appearance when running samples on the QIAxcel System and QIAxcel Advanced. Make sure QX DNA Dilution buffer has been used to dilute your samples. Dilute your samples 1:2 in QX DNA Dilution buffer and re-analyze.

FAQ ID -1837