QIAsymphony DSP Circulating DNA Kit

For automated purification of ccfDNA from 1-96 samples using the QIAsymphony SP

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QIAsymphony DSP Circulating DNA Kit (192)

Cat. No. / ID: 937556

Reagent cartridges, accessories and proteinase K vials for 192 preps of 2000 µl or 4000 µl each.

8.964.000,00 ₩

Quantity

The QIAsymphony DSP Circulating DNA Kit is intended for in vitro diagnostic use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

For in vitro diagnostic use
Lot-to-lot traceability
Produced under GMP manufacturing conditions

Product Details

The QIAsymphony DSP Circulating DNA Kit provides reagent cartridges and reagents for fully automated and simultaneous purification of human circulating cell-free (ccf) DNA from human plasma and urine using the QIAsymphony SP instrument. Protocols are available for 2 and 4 ml sample volumes.

Principle

QIAsymphony technology combines the speed and efficiency of anion exchange-based nucleic acid purification with the convenient handling of magnetic particles (see figure “ Schematic diagram of the QIAsymphony SP principle.”).

The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. These steps are repeated several times during sample processing The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously.

See figures

Efficient processing of magnetic particles.

Procedure

The QIAsymphony SP makes automated sample preparation easy and convenient. Samples, reagents and consumables, and eluates are separated in different drawers. Simply load samples, proteinase K, reagents provided in special cartridges, and preracked consumables in the appropriate drawer before a run. Start the protocol and remove purified DNA from the “Eluate” drawer after processing. Refer to the user manuals supplied with your instrument for operating instructions.

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 3 steps: bind, wash, and elute (see figure “ QIAsymphony DSP Circulating DNA procedure.”). The user can choose between different sample input volumes.

See figures

QIAsymphony DSP Circulating DNA procedure

Applications

ccfDNA purified using the QIAsymphony DSP Circulating DNA Kit and the QIAsymphony SP is ready for use in a wide range of downstream applications, including NGS, digital, multiplex and quantitative real-time PCR.

Supporting data and figures

Efficient processing of magnetic particles.

The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.

Resources

Improvements made to your QIAsymphony DSP Circulating DNA Kit

Download Safety Data Sheets for QIAGEN product components.

FAQ

At least 2.4 ml sample for the 2 ml protocol or 4.5 ml for the 4 ml protocol should be loaded.

Loading smaller volumes increases the risk of “unclear” flagged samples. As described in the corresponding labware list, volumes of 1.4–2.4 ml and 3.5–4.5 ml, respectively, will be processed but flagged “unclear” (“Enable less sample” mode).

Loading volumes less than recommended increases the risk that samples are not transferred (“invalid” flagging). If the sample volume is insufficient, add PBS to the required sample volume before loading the sample.

When using FIX labware (instrument does not perform liquid-level detection and reporting), load at least 2.1 ml for the 2 ml protocol and at least 4.1 ml for the 4 ml protocol. Because the instrument does not detect sample volumes using FIX labware, insufficient volumes might result in bubble formation during sample transfer and/or the subsequent binding step. If the sample volume is insufficient, add PBS to the required sample volume before loading the sample.

FAQ ID - 3701