QIAamp DNA Kits

For isolation of genomic, mitochondrial, bacterial, parasite or viral DNA

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QIAamp DNA Micro Kit (50)

Cat. No. / ID: 56304

For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)

7.161,00 MX$

KitAccessories

QIAamp DNA Kit

QIAamp DNA Accessory Set

Column type

Micro

Mini

Mini QIAcube

Quantity

QIAamp DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Rapid purification of high-quality DNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors
Automated purification of 1–12 samples on the QIAcube Connect

Product Details

QIAamp DNA Kits provide silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. DNA purification from 1–12 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on the QIAcube Connect.

QIAamp DNA Mini standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAamp DNA Mini protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.

Performance

DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR"). Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research.

The QIAamp DNA Mini Kit yields DNA sized up to 50 kb (see figure " Purification of up to 50 kb genomic DNA"). DNA of this length denatures completely and has the highest amplification efficiency. Yields of nucleic acids or DNA depend on the starting material (see table “Typical yields with the QIAamp DNA Mini Kit”). DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping and pharmacogenomic research.

Sample	Amount	Total nucleic acid yields (µg)*	DNA yields (µg)†
Blood	200 µl	4–12	4–12
Buffy coat	200 µl	25–50	25–50
Cells	10⁶	20–30	15–20
Liver	25 mg	60–115	10–30
Brain	25 mg	35–60	15–30
Lung	25 mg	25–45	5–10
Heart	25 mg	15–40	5–10
Kidney	25 mg	40–85	15–30
Spleen	10 mg	25–45	5–30

The QIAamp DNA Mini QIAcube Kit yields DNA sized up to 50 kb: DNA of this length denatures completely and has the highest amplification efficiency. The purified DNA may be used in a number of applications, including:

Viral research
Bacterial research
Fungal research
Cancer research
Human genetic testing research
Paternity testing
Forensic analysis

The QIAamp DNA Mini QIAcube Kit simplifies DNA isolation from tissue samples with fast spin-column procedures. The kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings (see figure " Significant time savings").

See figures

Efficient purification of DNA from small sample sizes.

Laser microdissection PCR.

Purification of up to 50 kb genomic DNA.

Significant time savings with dedicated QIAamp QIAcube Kits

Principle

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.

The QIAamp DNA Mini Kit simplifies DNA isolation from tissue samples with fast spin-column or vacuum procedures. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures.

During the QIAamp DNA purification procedure, DNA binds specifically to the QIAamp MinElute or QIAamp silica-gel membrane while contaminants pass through. No phenol-chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures.

QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

Procedure

The QIAamp DNA Micro Kit simplifies DNA isolation from small samples with a fast spin-column procedure (see flowchart " QIAamp DNA Micro procedure"). The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl. The QIAamp DNA Micro Kit can also be automated on the QIAcube Connect.
Optimized buffers and enzymes in the QIAamp DNA Mini Kit lyse samples, stabilize nucleic acids and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.
No mechanical homogenization is necessary as the tissues are lysed enzymatically. The convenient spin-column procedure means that hands-on preparation time is only 20 minutes (lysis times differ according to the sample source). Samples can be processed using either a microcentrifuge or, if blood or other body fluids are being processed, using the QIAvac 24 Plus. In addition, the rigorous lysis procedure employed makes the QIAamp DNA Mini Kit ideal for purification of genomic DNA from bacteria or parasites. To further reduce hands-on time, genomic DNA purification may be automated on the QIAcube. The QIAamp DNA Accessory Set A provides the additional buffers and reagents needed for isolation of genomic, mitochondrial, bacterial, parasite or viral DNA using the QIAcube with 12 x QIAamp DNA Mini Kits (50).

Vacuum processing

Blood or other body fluids can be processed by vacuum, instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp Mini spin columns are accommodated on the QIAvac 24 Plus manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.

Automated DNA isolation on the QIAcube Connect

The dedicated QIAamp DNA Mini QIAcube Kit is designed with preloaded spin columns and elution tubes in rotor adapters for QIAcube instruments, eliminating the risk of errors due to incorrect loading of rotor adapters. The dedicated kit is tailored to QIAcube requirements, reducing waste.
A simple protocol (lyse, bind, wash and elute) and an automated protocol on the QIAcube Connect requires minimal user interaction. The award-winning QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run. The QIAcube Connect and the dedicated QIAamp DNA Mini QIAcube Kit provide a winning combination for fast, easy and convenient DNA purification.

See figures

QIAamp DNA Micro procedure.

Applications

The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including small volumes of blood, blood cards, urine, small tissue samples, including laser microdissections.

The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, including muscle, liver, heart, brain, bone marrow and other tissues, swabs (buccal, eye, nasal, pharyngeal and others), CSF, blood, body fluids and washed cells from urine. DNA can be purified from up to 25 mg tissue or from up to 200 µl fluid in 20 minutes, and eluted in 50–200 µl.

The QIAamp DNA Mini QIAcube Kit is for automated isolation of genomic, mitochondrial, parasite or viral DNA on the QIAcube Connect. Sample sources include tissues, buccal swabs, CSF and washed cells from urine.

Comparison of QIAamp DNA Kits
Features	QIAamp DNA Micro Kit	QIAamp DNA Mini Kit
Applications	Real-time PCR, STR analysis, LMD-PCR	PCR, Southern blotting
Elution volume	20–100 µl	50–200 µl
Format	Spin column	Spin column
Main sample type	Whole blood	Whole blood, tissue, cells
Processing	Manual (centrifugation or vacuum)	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA, mitochondrial DNA	Genomic DNA, mitochondrial DNA, bacterial DNA, parasite DNA, viral DNA
Sample amount	1–100 µl	200 µl/25 mg/5 x 106
Technology	Silica technology	Silica technology
Time per run or per prep	30 minutes	20 minutes
Yield	<3 µg	4–30 µg

Supporting data and figures

Exemplary size distribution using QIAamp DNA Mini Kit (Cells)

Exemplary size distribution of DNA from cells using the QIAamp DNA Mini Kit. Samples were analyzed with the Femto Pulse System and signals were normalized to maximum peak.

Resources

For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA

Second edition — innovative tools

Download Safety Data Sheets for QIAGEN product components.

The protocol can be used for fresh or frozen semen samples with equal efficiency. Frozen samples must be thawed thoroughly before use. Please note that lysis time will vary depending on the size and density of the source material.

These procedures have been used successfully for isolation of genomic DNA from Aspergillus and Candida species, from both fungal cultures and blood.

This protocol has only been tested with ‘soft’ tissues (e.g., liver, spleen, thymus, heart, kidney, and brain) and may not work with ‘hard’ tissues (e.g., bone, teeth, and skin).

For parallel preparation of genomic, bacterial, or viral DNA or viral RNA from more than 24 samples, we recommend using QIAamp® Spin Columns with 4-6 ml collection tubes. Any tube with an inner diameter of 9-10 mm (outer diameter 11-12 mm) is suitable.

For QIAamp DNA Mini Kit (50) plus QIAamp DNA Accessory Set A, cat. no. 1048145, or QIAamp DNA Mini Kit (250) plus QIAamp DNA Accessory Set B, cat. no. 1048146

FAQ

Even though we have not tested this, we assume that both the QIAamp DNA Micro Kit and the AllPrep DNA/RNA Mini Kit will work to isolate DNA from mitochondria separated with the Qproteome Mitochondria Isolation Kit.

FAQ ID -1188

Yes, we have the following protocols:

Isolation of genomic DNA from compact bone using the QIAamp DNA Mini Kit (QA02)
Isolation of genomic DNA from compact bone using the DNeasy Blood & Tissue Kit (DY01)
Purification of genomic DNA from bones using the QIAamp DNA Micro Kit (QA39)
Purification of archive-quality DNA from bone fragments using the Gentra Puregene Tissue Kit (PG38).

FAQ ID -908

Yes, we have the following protocols:

Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; vacuum procedure (QA19v)
Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; spin procedure (QA19s)
Isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit; spin procedure (DY07)

FAQ ID -917

For DNA isolation using the QIAamp DNA Mini, or the DNeasy Blood & Tissue Kit, we recommend using carrier DNA when expected yields are below 10 ng. If possible, carrier DNAs such as poly-dA, poly-dT or poly-dA:dT should be used. Other carrier DNAs such as herring sperm DNA may interfere with subsequent PCR by binding primers nonspecifically.

Please note that poly-dA may interfere with oligo-dT primers, and, in this case, a different carrier DNA should be used. The concentration of carrier DNA should be at least 10 µg/ml. Optimal amounts need to be determined empirically for each application. The size distribution of carrier DNAs is typically in the range of 100 bp to 10 kb.

FAQ ID -100

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560

Yes, please follow the User-Developed Protocol 'Detection of Bordetella pertussis DNA by PCR' (QA14). The procedure is for use with the QIAamp DNA Mini Kit. Please contact Technical Service for this protocol.

FAQ ID -914

Fragments of approximately 200 bp can be isolated with good recovery. Smaller fragments can also be isolated but the recovery will be reduced with decreasing fragment lengths.

Yes, please follow either of the User-Developed Protocols:

'Acetyl Cysteine (NALC) treatment of viscous samples' (QA13). This protocol is for use with the QIAamp DNA Mini Kit.
'Acetyl Cysteine (NALC) treatment of viscous samples using the DNeasy Blood & Tissue Kit' (DY06).

FAQ ID -913

The expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit is approximately 10 ug of DNA per 2x 10⁹ bacterial cells. Note that yields of genomic DNA will vary depending on bacterial strain, quality of the starting material, growing conditions, and the amount of material processed.

FAQ ID -632

Yes, please follow either of the User-Developed Protocols:

'Isolation of DNA from soft tissues using the TissueLyser and QIAamp DNA Mini Kit' (QA31), for use with the QIAamp DNA Mini Kit
Purification of total DNA from soft tissues using the TissueLyser and the DNeasy Blood & Tissue Kit' (DY11), for use with the DNeasy Blood & Tissue Kit

FAQ ID -924

If you have optimized the lysis conditions for a specific sample type, you can lyse the sample in your lysis buffer, and follow the Supplementary Protocol 'Purification of total DNA from crude lysates using the DNeasy Blood & Tissue Kit' (DY15), or the corresponding protocol in the Appendix of the QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook.

The expected DNA yields from different tissues using the QIAamp DNA Mini Kit are as follows:

Sample	Nucleic acid yield without RNase A treatment (ug)	DNA yield with RNase A treatment (ug)
Blood (200 ul)	4–12	4–12
Buffy coat (200 ul)	25–50	25–50
Cultured cells (5 x 10e6)	20–30	15–20
Liver (25 mg)	60–115	10–30
Brain (25 mg)	35–60	15–30
Lung (25 mg)	8–20	5–10
Heart (25 mg)	25–45	5–10
Kidney (25 mg)	40–85	15–30
Spleen (10 mg)	25–45	5–30

DNA was purified with QIAamp Kits following standard protocols.

FAQ ID -45

Alcohol precipitation is commonly used for concentrating, desalting, and recovering nucleic acids. Since less alcohol is required for isopropanol precipitation, this is the preferred method for precipitation of DNA from large volumes. In addition, isopropanol precipitation can be performed at room temperature, which minimizes co-precipitation of salt that interferes with downstream applications.

Procedure

Adjust the salt concentration, for example, with sodium acetate (0.3 M, pH 5.2, final concentration) or ammonium acetate (2.0–2.5 M, final concentration).
Add 0.6–0.7 volumes of room-temperature isopropanol to the DNA solution and mix well.
Centrifuge the sample immediately at 10,000–15,000 x g for 15–30 min at 4°C
Carefully decant the supernatant without disturbing the pellet.
Wash the DNA pellet by adding 1–10 ml (depending on the size of the preparation) of room-temperature 70% ethanol. This removes co-precipitated salt and replaces the isopropanol with the more volatile ethanol, making the DNA easier to redissolve.
Centrifuge at 10,000–15,000 x g for 5–15 min at 4°C.
Carefully decant the supernatant without disturbing the pellet.
Air-dry the pellet for 5–20 min (depending on the size of the pellet).
Redissolve the DNA in a suitable buffer.

Tip: Use a buffer with a pH of 7.5–8.0, as DNA does not dissolve easily in acidic buffers. Often distilled water can have an acidic pH. The addition of EDTA protects the DNA from DNase digestion.

Tip: High-molecular-weight DNA, such as genomic DNA, should be redissolved very gently to avoid shearing. If the DNA pellet does not dissolve easily, heat at 55°C for 1–2 h with gentle shaking.

FAQ ID -2953

Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
Mix, and store at –20°C for at least 1 h to precipitate the DNA
Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min
Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
Allow the DNA pellet to air-dry
resuspend the DNA in a suitable volume of sterile TE buffer or distilled water

FAQ ID -305

Protocols for automated DNA extraction from saliva stabilized with the PAXgene Saliva Collector are available for the QIAsymphony DNA Midi Kit with the QIAsymphony SP instrument or the QIAamp DNA Mini Kit with the QIAcube (Classic and Connect).
For manual extraction, supplementary protocols are available for the QIAamp DNA Mini and Gentra Puregene Cell Kits (see resources section).

3821

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.

FAQ ID -374

When purifying small amounts of DNA using silica technology, the addition of carrier RNA or DNA enhances the recovery of DNA. Carrier prevents the small amount of target nucleic acid present in the sample from being irretrievably bound. Other typical “precipitation” carriers, such as glycogen, cannot be used. The carrier used must be a nucleic acid, and of a large enough size (>200nt) to bind to the silica membrane.

FAQ ID -473

QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.

FAQ ID - 3447

Yes, we have the following protocols:

Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 1 (QA03), long procedure
Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 2 (QA04), short procedure
Purification of total DNA from animal sperm using the DNeasy Blood and Tissue kit; protocol 1 (DY02), long procedure
Purification of total DNA from animal sperm using the DNeasy Blood and Tissue kit; protocol 2 (DY03), short procedure
Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit (QA40).

FAQ ID -909

The size of DNA obtained with QIAamp DNA Mini and QIAamp Blood Mini kit ranges between 100 bp and 50 kb, with 30 kb fragments predominating. The 30 kb fragments are a good starting point for most of the library preparations. Furthermore, the purified DNA is free of protein, nucleases, and other contaminants and inhibitors, and therefore is suitable for NGS.

FAQ ID - 3518

Yes, we have the following protocols:

Isolation of genomic DNA from fungi (culture and blood) using the QIAamp DNA Mini Kit (QA09).
Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN Genomic-tip (QG08).

FAQ ID -911

A vial of Protease with 7.5 Anson units (AU) contains approximately 167 mg protein (45 mAU/mg protein). If the content of the vial is diluted in 7 ml, the concentration would roughly be 24 mg/ml.

FAQ ID - 3520

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728

In QIAGEN labs, we have isolated DNA from a minimum of 1000 cells or 1 ul of whole blood sample. The expected yield from 1 ul of whole blood with 4000-7000 leukocytes is 30 ng of genomic DNA. The expected yield of genomic DNA from a single eukaryotic cell is 6 pg. However, please bear in mind that for these small quantities, we would recommend the QIAamp DNA Micro kit instead.

FAQ ID - 3516

Yes. We have used the QIAamp DNA Blood Mini Kit to purify DNA fragments as small as 168 base pairs. Our product profile for this kit shows a picture of the apoptotic banding pattern obtained after storage of blood samples at 4°C for extended periods of time prior to isolating DNA.

FAQ ID -149

The initial centrifugations are performed at 6000 x g simply to reduce centrifuge noise. The final spin with the ethanolic wash buffer and the optional drying spin are both done at full speed to enhance ethanol drying from the spin column. Earlier centrifugation steps can also be performed at full speed, and this will not negatively affect DNA yield or quality.

FAQ ID -474

Dedicated QIAcube kits are the RNeasy Mini QIAcube kit, QIAamp DNA Mini QIAcube Kit , the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit.

See: http://www.qiagen.com/Products/Catalog/Automated-Solutions/Sample-Prep/QIAcube#orderinginformation for a virtual demonstration on how to use the QIAcube.

FAQ ID -2337

The composition of Buffer AE is:

10 mM Tris-Cl
0.5 mM EDTA; pH 9.0.

FAQ ID -730

Yes, please follow the User-Developed Protocol 'Purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit' (QA43).

Note: The maximum amount of cells that can be used with this protocol has not been thoroughly tested. However, we would suggest using no more than 1 x 10⁶ cells.

FAQ ID -1547

Tissue is more difficult to lyse than cells in a blood sample. Therefore an additional incubation step in buffer ATL containing Proteinase K is included in the Tissue Protocol to achieve complete sample lysis. Blood cells will directly lyse by incubation in Buffer AL containing QIAGEN Protease. Buffer AL is required in both protocols to ensure appropriate conditions for DNA binding to the silica membrane. For further details on the protocols, please refer to the QIAamp DNA Mini and QIAamp DNA Blood Mini Kit Handbook.

FAQ ID -633

QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.

To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.

FAQ ID -315

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618

Yes, please follow the User-Developed Protocol 'Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit' (QA40).

FAQ ID -926

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

EDTA chelates divalent cations which are required for nuclease activity. While the genomic DNA (gDNA) extracted using QIAGEN products, should not have any nuclease activity, it is possible to introduce nucleases during repeated long-term access of the DNA. EDTA helps to prevent any nuclease activity introduced after the genomic DNA extraction procedures.

However, if the gDNA is stored frozen at -20oC or -80oC, nuclease activity is much reduced. It may be possible to leave EDTA out of the storage buffer without negative consequences when samples are kept under these conditions, and when repeated freeze-thaw cycles are avoided.

We do recommend however that gDNA be stored in a neutral to a slightly basic buffered solution (e.g. 10 mM Tris-Cl pH 8.5 to 9.0) to prevent DNA degradation by acid hydrolysis. Note that deionized water mostly has an acidic pH.

FAQ ID -754

Yes, please follow the Supplementary Protocol 'Purification of REPLI-g amplified DNA using the QIAamp DNA Mini Kit' (RG14).

FAQ ID -1545