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PCR Anti-Inhibitor (100)
Cat no. / ID. RP50
100 μL of PCR Anti-Inhibitor, liquid
Storage temperature: -20°C
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Quantity
100 uL
500 uL
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The PCR Anti-Inhibitor is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM
Features
- Possesses a mixture of alkaline
- Eliminates inhibitors found in a DNA template isolation
Product Details
PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins which counteracts substances inhibiting the PCR reaction. Adding PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originating in a DNA template isolation.
Performance
| Assay | Specification |
|---|---|
| Functionality | Passed |
| DNase contamination | None detected |
Principle
Quality Control
DNase activity was evaluated by incubating 1 μg of DNA with 1 μL of the enzyme for 4 hours at 37°C. Results are visualized on an ethidium bromide-stained agarose gel. The product was extensively tested in PCR reaction.
Usage
Add the PCR Anti-inhibitor to the PCR mixture in a 1:50 volume ratio, e.g., add 1 μL of the PCR Anti- inhibitor to 50 μL PCR reaction.
Applications
This is used for application such as:
- PCR with difficult DNA templates isolated from samples such as urine, saliva, sputum, blood, cell swabs, cerebrospinal fluid, biopsies, etc.
Resources
Certificates of Analysis (1)