Virtual Demo
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QIAgility HEPA/UV (w/o PC) System
Cat. No. / ID: 9001908
Robotic workstation for automated PCR setup (with UV light and HEPA filter), comes with QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts. Does not include a laptop.
Contains
Without PC
With PC
Service Options
System
Priority
PrioPLUS
The QIAgility, if used in combination with QIAGEN kits indicated for use with the QIAgility instrument, is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAgility instrument is used with kits other than QIAGEN kits, it is the user’s responsibility to validate the performance of such product combination for any particular application.
The QIAgility is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Virtual Demo
Explore the virtual tour to learn more and see this product in action.
Features
- Automated PCR setup in all formats
- Convenient, easy-to-use software
- Pre-optimized experiment setup
- Standardized results and increased productivity
- Elimination of manual pipetting steps
Product Details
The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience. The software provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility the QIAgility is operated via a laptop computer.
Explore the virtual demo to learn more about the QIAgility.
QIAgility operates in conjunction with QIAgility Accessories and QIAgility Consumables.
Performance
The QIAgility fully automates proven QIAGEN PCR kits for use with various workflows, and offers pure pipetting precision, enhanced safety features, and exceptional reliability and reproducibility.
Unmatched pipetting precision with QIAgility
The QIAgility instrument operated by the QIAgility Setup Manager Software demonstrates exceedingly high accuracy and precision of dispensed volumes as verified by the Artel MVS system (see figure “ Exceptional pipetting precision and accuracy spanning over a broad volume range”). These results indicate that the QIAgility can produce automated, high-performance liquid dispensing results, while avoiding common errors that can be associated with manual pipetting and thereby reducing the time to result.
Regardless of whether you pipet water or QIAGEN ready-made reaction mix to set up a PCR experiment, the QIAgility instrument can dispense volumes that are highly precise and also generate significantly reproducible replicates (see figure “ Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions”).
High performance PCR setup using QIAgility
A QuantiNova SYBR Green PCR assay setup was performed manually and automated in parallel using the QIAgility along with its Setup Manager Software. The amplification run was done on the Rotor-Gene Q and resulting data analyzed using the Q-Rex Software. Consistent results were obtained from both manual and automated reaction setup using the same template dilutions (see figure “ Comparison of manual and automated assay setup using series dilutions”).
Exceptional linearity of QIAgility
QIAgility exhibits excellent linearity and assures accurate and reliable use of sample for an assay. To assess the linearity of the system, pure gDNA was serially diluted using the QIAgility and measured for their concentrations on the QIAxpert. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAgility (see figure “ High system linearity of QIAgility”).
See figures
Principle
Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that are typically prone to human error.
Liquid-handling tasks are performed by the single-channel pipetting head, which provides fast transfer of liquids with various types of plasticware. For example, 96 samples with the QuantiFast SYBR Green PCR Kit with 2 standard series can be setup in approximately 45 minutes. The worktable features a flexible layout and supports almost all kinds of cyclers and labware, giving high application versatility. Dynamic liquid-level sensing, facilitated by conductive filter tips offers maximum safety control.
The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. A built-in sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. The UV light ensures effective worktable decontamination and the HEPA filter provides positive clean air pressure throughout PCR setup.
QIAgility is a compact benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. For added flexibility, the QIAgility comes with a laptop computer. To reduce your work load, comprehensive run reports containing all run information are generated automatically during each protocol.
Procedure
Quick and easy setup of your PCR experiment with the new Setup Manager Software
Let the QIAgility do the pipetting and save your valuable time
Let the QIAgility do the pipetting and save your valuable time
Applications
The instrument is highly suited for laboratories performing PCR and RT-PCR reactions with applications in:
- Gene expression analysis
- Genotyping
- Cloning
- Pathogen detection
- Food and veterinary testing
The new QIAgility Setup Manager Software offers a complete solution for qPCR setup, sample normalization, CE loading, certain liquid handling, as well as sample pooling. The software includes Gene Expression, Pathogen Detection, Sample Pooling and Generic PCR plug-ins. The Gene Expression and Pathogen Detection plug-ins are optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiNova, miRCURY and RT² kits. The Generic PCR plug-in allows flexible qPCR setup, liquid handling and support for the whole HID workflow.
Check the instrument operating software and protocol files under product resources for more information.
Services
Is your plate or tube not supported in the QIAgility software? Contact QIAGEN Technical Services to request a new plate or tube to suit your workflow.
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Learn more about trade-in/trade-up opportunities.
Supporting data and figures
Exceptional pipetting precision and accuracy spanning over a broad volume range
The Artel MVS system was employed to determine the pipetting precision of the QIAgility instrument operated by the QIAgility Setup Manager Software. The QIAgility dispensed the Artel solutions (selected according to the Artel MVS User Manual) into the Artel buffer. Three runs were performed on three QIAgility instruments that were used to pipet an entire volume range (1, 2, 5, 10, 20, 50, 100, and 200 µl), in replicates of 12. For each run and volume (n=12) the coefficient of variation (CV) was calculated to determine the pipetting precision of the QIAgility. The data shows the mean of the CV values (bars; in %) ± standard deviation (S.D.) of the CV values as error bars. The red lines depict the claimed specifications.
The indicated CV values (%) demonstrate that the QIAgility instrument performs within its claimed specifications and even better.
The indicated CV values (%) demonstrate that the QIAgility instrument performs within its claimed specifications and even better.
Specifications
| Features | Specifications |
|---|---|
| Accessories (supplied) | 2 x Adapter, 96 x 0.2 ml PCR Tubes 1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base 1 x Adapter, 72 x 0.1 ml Strip Tubes 1 x Master Mix Block, 5 x 5 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml 1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes 4 x Tip Rack Holder for 96-Tip Rack The adapters are intended to hold tubes or plates. |
| Conductive Filtered Tips | 50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing |
| Dimensions | Height: 45 cm (18 in.), instrument lid closed; Height: 86 cm (34 in.), instrument lid open; Width: 54 cm (21 in.); Depth: 63 cm (25 in.), without cables |
| Software | Supplied with unlimited user license. Free upgrades by Web download. |
| Pollution level | 2 |
| Temperature | Operating temperature, 15–30 °C (59–86°F) |
| Pipetting head | Single channel |
| Tip volume | 1–200 µl |
| HEPA filter | Preinstalled dual-cartridge HEPA filter system provides positive pressure clean air within the closed hood. |
| Liquid-level sensing | Minimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with QIAGEN Conductive Filtered Tips. |
| Communications | USB and RS232 |
| Consumables (supplied) | 10 racks of 50 µl Conductive Filtered Tips (960); 10 racks of 200 µl Conductive Filtered Tips (960); 25 x 5 ml tubes; 100 x 0.2 µl PCR tubes; 3 Tip Disposal Boxes |
| Electrical | 100–240 V AC, 250 VA, 50–60 Hz |
| Table capacity | 6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders 1 x Master Mix Block 1 x Reagent Block |
| Precision | CV <1%, 5–200 µl (dry-well transfer) CV <5%, 2–5 µl (wet-well transfer) CV <10%, 1 µl |
| Tip disposal | All used tips ejected externally — no waste accumulation on the worktable |
| Place of operation | For indoor use only |
| UV lamp | Preinstalled UV lamp for worktable decontamination |
| Humidity | Operating humidity: 40–70% |
| Notebook (supplied) | QIAgility comes with a notebook computer for added flexibility. |
| Warranty | 1-3 years warranty, depending on service package |
| Weight | 41 kg (90.2 lb) |
Service Plans
QIAgility, Core Agreement
Cat. No. / ID: 9245243
For the QIAgility, one on-site repair service and one on-site Preventive Maintenance or Inspection Service, including travel, labor and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during the Core Agreement period.
Resources
Instrument User Manuals (6)
Brochures & Guides (8)
Application Notes (2)
Protocol Files (1)
Operating Software (3)
Technical Information (3)
Validation Reports (1)
Safety Data Sheets (1)
Scientific Posters (1)
Certificates of Analysis (1)
FAQ
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
How can I decontaminate my QIAsymphony SP / AS system?
How do I transfer the QIAgility calibration settings to a different computer?
What do I do if the pipettor cannot pick up tips?
What do I do when QIAgility makes grinding noise?
Can I use a bar code scanner or reader on the QIAgility?
What file is required for reporting QIAgility issue?
What causes Error 131?
What do I do for error message “cannot detect liquid”?
What do I do if a red exclamation icon appears in the top-right corner of the schematic plate on the main software window?
What do I do if the pipettor hits the tip rack?
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
Can Vapor-Lock be used with samples processed on the QIAgility?
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
What sample tubes can I use on my QIAsymphony SP system?
Why do I do if the QIAgility software runs in virtual mode while the instrument is connected to the computer?
What do I do if tips are not ejected?