Products
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Features
- Internal control for detection of inhibition
- Quantitation using the 4 standards supplied
Product Details
The artus BK Virus RG PCR Kit and the artus BK Virus QS-RGQ Kit are ready-to-use molecular detection kits for real-time PCR on Rotor-Gene Q or Rotor-Gene 6000 instruments. The kits provide all necessary reagents for rapid and sensitive detection of BK virus specific DNA isolated from plasma and urine with quantitation for research applications. The artus BK Virus RG PCR Kit uses automated sample preparation and manual assay setup techniques. The artus BK Virus QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Principle
The artus BK Virus RG PCR Kit and the artus BK Virus QS-RGQ Kit are based on the amplification and simultaneous detection of a specific region of the BK virus genome using real-time PCR for research applications.
Each artus BK Virus Kit provides 4 BK virus quantitation standards (see figure " Quantitation of BK virus DNA"). Use of the standards enables quantitation of BK virus DNA. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical BK virus PCR is not reduced.
| Kit | artus BK Virus RG PCR Kit and artus BK Virus QS-RGQ Kit |
| Sample types | Plasma or urine |
| Amplicon | 274 bp region covering parts of the VP2 and VP3 genes |
See figures
Procedure
artus BK Virus Kits provide all necessary reagents optimized for reliable BK virus DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
Complete automated system from sample to BK virus detection
The QIAsymphony RGQ workflow solution for BK virus detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus BK Virus QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure " Integrated QIAsymphony RGQ system for BK virus detection").
Recommendations for viral DNA purification when using the artus BK Virus RG PCR Kit
For the artus BK Virus RG PCR Kit, we recommend using the EZ1 DSP Virus Kit.
See figures
Applications
The artus BK Virus RG PCR Kit enables rapid and sensitive detection of BK virus DNA from plasma or urine samples with highly accurate quantification on Rotor-Gene Q instruments for research applications.
The artus BK Virus QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from plasma or urine samples to BK virus detection and quantitation.
Supporting data and figures
Quantitation of BK virus DNA.
Quantitation of BK virus DNA.
Resources
Kit Handbooks (2)
Safety Data Sheets (1)
Certificates of Analysis (1)