Buffer RLT

For lysis of cells and tissues before RNA isolation

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Buffer RLT (220 ml)

Cat no. / ID. 79216

220 ml RNeasy Lysis Buffer

239,00 $

Quantity

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Buffer RLT is a lysis buffer for lysing cells and tissues prior to RNA isolation and simultaneous RNA/DNA/Protein isolation. When following RNeasy Plus or AllPrep DNA/RNA procedures, Buffer RLT Plus should be used.

Resources

Download Safety Data Sheets for QIAGEN product components.

Publications

Transcriptional regulation of uterine vascular endothelial growth factor during early gestation in a carnivore model, Mustela vison.

Lopes FL; Desmarais J; Ledoux S; Gévry NY; Lefevre P; Murphy BD;

J Biol Chem; 2006; 281 (34):24602-11 2006 Jun 21 PMID:16790435

FAQ

The exact composition of Buffer RLT is confidential. This buffer is a proprietary component of RNeasy Kits. Buffer RLT contains a high concentration of guanidine isothiocycanate, which supports the binding of RNA to the silica membrane. Buffer RLT can be purchased separately (cat. no. 79216)

Note: note that ß-mercaptoethanol should be added to Buffer RLT before use to effectively inactivate RNAses in the lysate (10 µl ß-Mercaptoethanol per 1 ml Buffer RLT).

FAQ ID -2793

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

Wird häufig dazu gekauft

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Solutions for Laboratory-Developed Tests

Buffer RLT

For lysis of cells and tissues before RNA isolation

Buffer RLT (220 ml)

Product Details

Resources

Publications

Transcriptional regulation of uterine vascular endothelial growth factor during early gestation in a carnivore model, Mustela vison.

FAQ

Wird häufig dazu gekauft

Buffer RPW (125ml)

Buffer RW1 (220 ml)

Buffer RPE (concentrate, 55 ml)

MagAttract Suspension G (13 ml)

Buffer EL (1000 ml)

96-Well Microplates MP (20)

Accessories Pack, QXT

Large 96 Rod-Cover (16)s

AdnaTest CTC-Select

QuantiNova Multiplex RT-PCR Kit (2500)

Adapter, Reagent Trough SBS Plate

Adapter, Reagent Trough R1 Position Plate

Buffer AW1 (concentrate, 242 ml)

RNeasy Mini Kit (50)

Buffer AVL (155 ml)

RNeasy Mini Kit (250)

Filter-Tips, 1000 µl (1024)

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Buffer RLT

For lysis of cells and tissues before RNA isolation

Buffer RLT (220 ml)

Product Details

Resources

Publications

FAQ

Wird häufig dazu gekauft