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One-Step Probe RT-qPCR Mix ROX (100)
Cat. No. / ID: AM09.1-100
100 rxns (20 μL)
Mu-MLV Reverse Transcriptase and RNase Inhibitor and ROX solution are included in separate tubes.
Components: 2x 1 mL One-Step Probe RT-qPCR Mix, 40 μL 50x High ROX solution, 40 μL 50x Low ROX solution, 10 μL RNase Inhibitor, 10 μL Reverse Transcriptase, 1.9 mL Nuclease-free water
Storage temperature: Store all components at -20°C. It does not lose its activity after eight successive thaw cycles. Aliquoting can be applied if necessary.
OEM Product
One-Step Probe RT-qPCR Mix ROX
One-Step Probe RT-qPCR Mix
Reactions
100
500
The One-step Probe RT-qPCR Mixes is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
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Features
- Detects low copies of RNA targets from a broad range of samples
- Exhibits consistent amplification across a wide dynamic range
- Eliminates non-specific amplification through precisely selected anti-Taq antibody
- Has a high efficiency in multiplex reactions
- Detects molecular targets accurately as fast as 40 minutes with two or three steps cycling profiles
Product Details
One-Step Probe RT-qPCR Mix is a convenient reaction mixture for fast and reliable quantitative Real-Time PCR using probes, including TaqMan ®, Scorpions ® and molecular beacon probes.
It was created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. It is the best choice for probe-based Real-Time PCR assays, including singleplex and multiplex gene expression studies.
Performance
| Assay | Specification |
|---|---|
| DNase contamination | None detected |
| RNase contamination | None detected |
Principle
Using high-affinity antibodies for hot-start polymerase ensures higher specificity by reducing the formation of primer-dimer structures. It allows for a more comprehensive dynamic range by removing competition for reaction reagents. It also leads to higher sensitivity and reproducibility.
Precisely optimized buffer components ensure optimal reverse transcriptase and hot-start polymerase activity conditions. Additionally, RNase Inhibitor protects RNA from unspecific RNases.
The One-Step Probe RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly used Real-Time PCR platforms.
Procedure
Quality Control
One-Step Probe RT-qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of DNase and RNase contamination.
Applications
This is used for applications such as:
- RT-qPCR
- Gene expression analysis and genetic profiling
- miRNA profiling and quantification
- Mass screening and RNA viral pathogen detection
- Characterization of genetically modified organisms (GMO)
Resources
Safety Data Sheets (1)
Certificates of Analysis (1)