Configurer sur GeneGlobe
Trouvez ou créez les dosages et panels spécifiques qu’il vous faut pour identifier vos cibles biologiques d’intérêt.
QIAseq Multimodal Custom Panel (96)
N° de réf. / ID. 333955
Kit containing ALL reagents (except indexes) for multimodal (DNA and RNA) sequencing; Custom panel for a total of 192 reactions to process 96 samples
Configurer sur GeneGlobe Pour voir le prix
panelindex
Panel
Index
Le règlement REACH (CE 1907/2006 Annexe XIV) s’applique aux substances réglementées que contient ce produit. L’utilisation de ce produit au sein de l’UE est soumise à une exemption (article 56(3)). Pour plus d’informations, consultez le règlement REACH ainsi que la fiche de données de sécurité de ce produit, que vous trouverez dans la section « Ressources » de cette page.
Les QIAseq Multimodal Custom Panels sont destinés aux applications de biologie moléculaire. Ces produits ne sont pas conçus pour le diagnostic, la prévention ou le traitement des maladies.
Configurer sur GeneGlobe
Trouvez ou créez les dosages et panels spécifiques qu’il vous faut pour identifier vos cibles biologiques d’intérêt.
Caractéristiques
- Complete system to go from total nucleic acids to a sequencing-ready library using a single-day workflow
- Error correction with Unique Molecular Indices (UMIs) to enhance NGS panel sensitivity
- QIAseq enrichment technology overcomes the limitations of traditional amplicon approaches on amplicon size and primer multiplexing
- Unique Dual Indices (UDIs) to reduce sample index hopping
- Sample to Insight solution for consolidated targeted DNA and RNA sequencing
Détails produit
QIAseq Multimodal Panels have been developed for consolidated targeted DNA and RNA enrichment and analyses. Unlike other available approaches, QIAseq Multimodal Panels do not require 2 separate workflows for DNA and RNA analysis – saving time and conserving samples that are of limited availability.
Custom panels can be designed to target exonic regions of genes, hotspots or SNPs, intronic and promoter regions, known gene fusions based on characterized breakpoints, or to discover novel fusions.
Need a quote for your research project or would you like to discuss your project with our specialist team? Contact Us
Principe
Recent advances in NGS chemistries, platforms, and bioinformatics pipelines are enabling users to efficiently interrogate biological samples for changes in DNA and RNA. Other available approaches, however, require the use of 2 separate workflows to prepare libraries from separate DNA and RNA isolates. Limitations of such approaches include:
- Large amounts of sample material required for generating sufficient amounts of input DNA and RNA for multiple workflows
- Added complexity of deriving integrated insights from results of different technical approaches, each with its own innate bias
- Inefficient use of resources
- Long turnaround time
To overcome the limitations associated with current approaches, QIAseq Multimodal Panels start with total nucleic acids (or DNA + RNA) and prepares UDI-containing, Illumina-compatible targeted DNA and RNA libraries using a single-day, consolidated workflow. In addition, QIAseq Multimodal Panels have been designed for use with low-yield and poor-quality biological samples.
Procédure
Workflow of the QIAseq Multimodal Panels
The QIAseq Multimodal Panel workflow can be used to prepare sequencing-ready libraries in a single day. The library insert size is approximately 150 bp, making the QIAseq Multimodal Panels highly compatible with low-quality samples, such as FFPE samples.
Robust detection of DNA and RNA biomarkers
QIAseq Multimodal Panels can be used to reliably detect DNA and RNA biomarkers using a consolidated workflow from total nucleic acids. The ability of QIAseq Multimodal Panels to simultaneously detect DNA and RNA biomarkers has been benchmarked against two separate workflows (namely, QIAseq Targeted DNA Pro and QIAseq Targeted Fusion XP).
Applications
QIAseq Multimodal Panels can be used to interrogate different types of biomarkers using a consolidated workflow from total nucleic acids, which can be isolated using dedicated sample isolation protocols that have been developed specifically for the QIAseq Multimodal Panels.
From DNA:
- Single nucleotide variants (SNVs)
- Insertions and Deletions (InDels)
- Copy number variants (CNVs)
From RNA:
- Fusions
- Exon skipping events
- Gene expression levels
Ressources
Safety Data Sheets (3)