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QIAseq miRNA Library QC Spike-ins
Cat. No. / ID: 331535
Formerly Exiseq NGS Spike-In Kit #800100. 52 QIAseq miRNA Library QC Spike-ins, sufficient for up to 500 samples; nuclease-free water
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Kit
QIAseq miRNA Library QC Spike-ins
QIAseq miRNA Library QC qPCR Assay Kit
QIAseq miRNA Library QC PCR Panel Kit
QIAseq miRNA Library QC PCR Panel and Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Unique qPCR-based sample QC of miRNA/small RNA samples prior to NGS
- Essential for challenging samples with low RNA content, such as biofluids
- LNA miRNA PCR Assays in ready-to-use PCR panels
- Compatible with all major qPCR instruments
- Comprehensive set of 52 RNA spike-ins, spanning a wide range of concentrations
- Thorough post-sequencing assessment of NGS linearity and reproducibility
Product Details
QIAseq miRNA Library QC PCR Assays and Arrays enable rigorous quality control of both sample quality before NGS library preparation as well as NGS performance post-sequencing. Amplification of the QIAseq miRNA Library QC Spike-ins – a comprehensive set of spike-ins added during RNA isolation or to isolated RNA – allows assessment of the reproducibility and linearity of the NGS reads mapped to these exogenous sequences, ensuring optimal miRNA sequencing results.
QIAseq miRNA Library Kits are recommended for gel-free library construction miRNA, piRNA and small RNA on Illumina and Thermo Fisher Scientific NGS instruments.
Principle
The QIAseq miRNA Library QC PCR Panel Kit assesses the quality of RNA isolation for small RNA next-generation sequencing (NGS) by providing spike in controls with a qPCR panel that allows researchers to monitor for reproducibility between miRNA isolations, the presence of enzymatic inhibitors and nucleases, sample assessment for hemolysis (important for serum and plasma miRNA identification) and a thorough QC of the NGS data by assessing the reproducibility and linearity of the reads mapped to these exogenous sequences.
The kit is compatible with many samples types but is especially useful for challenging samples with low RNA content such as serum, plasma, urine and other biofluids.
The kit is compatible with many samples types but is especially useful for challenging samples with low RNA content such as serum, plasma, urine and other biofluids.
| QIAseq miRNA Library QC PCR Panel Kit | QIAseq miRNA Library QC Spike-Ins | |
|---|---|---|
| Compatible with biofluid samples | ✓ | ✓ |
| Compatible with cells/tissue | — | ✓ |
| Evaluate RNA yield | ✓ | — |
| Evaluate miRNA/small RNA quality | ✓ | — |
| Evaluate library quality | ✓ | ✓ |
| Evaluate NGS reproducibility | ✓ | ✓ |
| Evaluate technical outliers | ✓ | ✓ |
Procedure
Addition of QIAseq miRNA Library QC Spike-Ins during RNA isolation enables monitoring of the comparability and reproducibility of the whole process from RNA isolation to NGS data analysis.
QIAseq miRNA Library QC Spike-Ins can also be added directly to RNA samples before small RNA library preparation. For more accurate ratios of spike-ins vs. endogenous miRNAs in other sample types or when using isolated RNA samples, experimental titration of QIAseq miRNA Library QC Spike-Ins is recommended.
The protocol consists of 5 steps:
Supporting data and figures
Useful for a wide range of sample types.
The QIAseq miRNA Library QC PCR Panel can be used with a wide range of samples, as the miRNAs on the panel are expressed in many different sample types. In this example, human serum, plasma, urine, colorectal cancer (FFPE) and mouse brain (fresh frozen) samples were analyzed.
Resources
Scientific Posters (1)
Safety Data Sheets (1)
Package Insert (4)
miRNA Research (2)
Kit Handbooks (1)
Quick-Start Protocols (1)
Certificates of Analysis (1)