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ipsogen Leukemia
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ipsogen Leukemia
ipsogen
RUNX1-RUNX1T1 Kit
ipsogen
RUNX1-RUNX1T1 Kit
采用real-time PCR对RUNX1-RUNX1T1(或AML1-ETO)融合基因转录本进行定量检测
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
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ipsogen
RUNX1-RUNX1T1 Kit (24)
目录编号 / ID.
675013
For 24 reactions: ABL Control Gene Standards, RUNX1-RUNX1T1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix RUNX1-RUNX1T1 Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
灵敏的定量检测试剂
对与ABL对照基因表达相关的转录本进行准确定量
符合EAC要求的标准化试剂,结果具有可重复性
卓越的质量保证,获得ISO 13485认证
即用型解决方案,便利的工作流程
产品详情
ipsogen
RUNX1-RUNX1T1 Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。
ipsogen
RUNX1-RUNX1T1 Kit中的试剂经过优化,能够对骨髓或外周血样本中的RUNX1-RUNX1T1(或AML1-ETO)融合基因转录本相对于ABL对照基因的表达的可靠、灵敏的相对定量检测。
绩效
为确保获得高检测灵敏度,
ipsogen
RUNX1-RUNX1T1 Kit经过优化,能够检测宽泛的线性范围内的ABL和RUNX1-RUNX1T1融合基因转录本(参见
Sensitive detection of RUNX1-RUNX1T1
和
ABL standards
)。
原理
ipsogen
RUNX1-RUNX1T1 Kit为即用型试剂盒,采用real-time PCR技术,检测RUNX1-RUNX1T1融合基因转录本。该试剂盒可扩增并检测特定的RUNX1-RUNX1T1融合基因转录本(参见
RUNX1-RUNX1T1 fusion gene transcript
和
ABL control gene transcript
),检测特异性、灵敏度和可重复性高。
ipsogen
RUNX1-RUNX1T1 Kit为ABL基因提供3个标准品稀释液,为RUNX1-RUNX1T1融合基因提供5个标准品稀释液。试剂盒中的引物探针对的设计符合欧洲抗癌协会(Europe Against Cancer,简称EAC)的推荐。
程序
第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。
ipsogen
RUNX1-RUNX1T1 Kit可对RUNX1-RUNX1T1融合基因和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
RUNX1-RUNX1T1 Ki能够对人类细胞中RUNX1-RUNX1T1基因转录本相对于ABL对照基因的表达的可靠、灵敏的定量检测。仅适用于科研。
辅助数据和图表
Sensitive detection of RUNX1-RUNX1T1 and ABL standards.
Detection of [
A
] RUNX1-RUNX1T1 standards (F1 to F5) with 10
1
, 10
2
, 10
3
, 10
5
, and 10
6
copies/5 μl and [
B
] ABL standards (C1, C2, C3) with 10
3
, 10
4
, and 10
5
copies/5 μl.
资源
试剂盒操作手册 (1)
(EN) - ipsogen FusionQuant Handbook
EN
PDF
(937KB)
安全数据表 (1)
Safety Data Sheets
EN
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
EN