QIAprep Spin Miniprep Columns
For purification of up to 20 μg molecular biology grade plasmid DNA
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QIAprep 2.0 Spin Miniprep Columns (100 spin columns)
Cat. No. / ID: 27115
For high-purity plasmid minipreps
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The QIAprep Spin Miniprep Columns is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
Product Details
The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
QIAprep Spin Miniprep standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. Learn more.
Performance
The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep Spin Column handling options A, B, and C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.
| Format: | Spin columns |
| Purification module: | QIAprep spin columns |
| Throughput: | 1–24 samples |
| Preparation time: | 24 minipreps in 30 minutes |
| Equipment required: | Microcentrifuge or vacuum manifold; fully automatable using the QIAcube |
| Lysate clearing: | Centrifugation |
| Capacity of column reservoir: | 800 µl |
| Minimum elution buffer volume: | 50 µl |
| Culture volume for high-copy plasmids: | 1–5 ml |
| Culture volume for low-copy plasmids/cosmids: | 1–10 ml |
Purified DNA can be used in restriction digestion (see figure " Complete digestion with various restriction enzymes").
See figures
Principle
QIAprep spin columns contain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediately ready to use - there is no need to precipitate, concentrate, or desalt.
Procedure
Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart " The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.
In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
See figures
Applications
The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:
- PCR
- Restriction digestion
- Ligation and transformation
- Sequencing
- Screening
Compatible with Gilson's TRACKMAN Connected system
QIAGEN has programmed QIAprep Spin Miniprep protocols into the TRACKMAN Connected system, which controls the Bluetooth-enabled PIPETMAN M Connected pipettes sold by Gilson.
TRACKMAN Connected guides researchers through the QIAprep Spin Miniprep protocols while automatically adjusting pipette settings. Each step of the protocol execution is recorded to accelerate reporting, because the system generates a comprehensive run report.
Supporting data and figures
The QIAprep procedure.
The QIAprep procedure.
Specifications
| Features | Specifications |
|---|---|
| Applications | Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation etc. |
| Samples per run (throughput) | 1–24 samples per run |
| Elution volume | 50 µl (minimal) |
| Processing | Manual (vacuum or centrifugation) |
| Yield | <20 µg |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Time per run or prep per run | <30 minutes |
| Technology | Silica technology |
| Culture volume/starting material | 1–10 ml culture volume |
Resources
User-Developed Protocols (4)
Scientific Posters (3)
Application Notes (1)
Application/Protocol Documents (1)
Supplementary Protocols (1)
Certificates of Analysis (1)