

Filter Result By Reset All Supplementary Protocols & Documentation Supplementary Protocols Supplementary Protocols 376 Application Notes Application Notes 140 User-Developed Protocols User-Developed Protocols 100 Vector Sequences & Maps Vector Sequences & Maps 66 Validation Reports Validation Reports 62 Supplementary Files Supplementary Files 10 Transfection Protocols Transfection Protocols 2 See all Show less Newsletter Subscribe to our newsletter Select topics of interest in My QIAGEN. Search Resources Bookmark Share more.. Search all resources Find protocols, product documentation, software, FAQs, videos, webinars, and more. Type in your search terms, and refine the results using the filters options on the left. Tip: Search with product name or catalog number to find all the resources related to your products! You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sort by category Sorted by relevance Isolation of genomic DNA from flies using the QIAGEN® Genomic-tip - (EN) Lysis time will vary depending on the size and density of the source material. The QIAGEN Genomic-tip will run slowly due to the high debris content in the nuclear fraction. The yield is about 0.3-0.4 µg DNA/fly. Found in: User-Developed Protocols Show details Isolation of genomic DNA from blood (erythrocyte lysis) using the QIAGEN® Genomic-tip 100/G - (EN) Found in: User-Developed Protocols Show details Isolation of genomic and viral DNA from lymphocytes using the QIAGEN® Genomic-tip - (EN) Found in: User-Developed Protocols Show details Purification of total DNA from viscous samples using the DNeasy® Blood & Tissue Kit with acetyl cysteine (NALC) pretreatment - (EN) Found in: User-Developed Protocols Show details Purification of total DNA from animal sperm using the DNeasy® Blood & Tissue Kit; protocol 1 - (EN) Found in: User-Developed Protocols Show details Purification of viral DNA from animal stool using the DNeasy® Blood & Tissue Kit - (EN) Found in: User-Developed Protocols Show details Purification of total DNA from animal sperm using the DNeasy® Blood & Tissue Kit; protocol 2 - (EN) Found in: User-Developed Protocols Show details Purification of total DNA from animal saliva using the DNeasy® Blood & Tissue Kit - (EN) Found in: User-Developed Protocols Show details Purification of total DNA from soft tissues using the TissueLyser and the DNeasy® Blood & Tissue Kit - (EN) Found in: User-Developed Protocols Show details Forensic post-PCR purification protocol using the MinElute PCR Purification Kit - (EN) Found in: User-Developed Protocols Show details Isolation of mRNA from adipose tissue using Oligotex® Direct mRNA Kits - (EN) The procedure has been successfully used for isolation of mRNA from adipose tissue by customers. Because of the nature of adipose tissue it cannot be used immediately in Oligotex Direct mRNA Protocols; simple centrifugation, however, eliminates the problem, and the procedure works fine. Found in: User-Developed Protocols Show details Regeneration of Oligotex® resin from the Oligotex mRNA Kit - (EN) The procedure has been used successfully by customers for regeneration of Oligotex resin from spin columns following elution of poly A⁺mRNA. Found in: User-Developed Protocols Show details For purification of Norovirus RNA from human stool samples using the BioSprint® 96 workstation - (EN) Found in: User-Developed Protocols Show details For purification of SBV RNA from bull semen using the BioSprint® workstations - (EN) Found in: User-Developed Protocols Show details For purification of DNA from stool samples using the BioSprint 15 DNA Blood Kit - (EN) Found in: User-Developed Protocols Show details For simultaneous purification of viral DNA and RNA from veterinary samples such as serum, plasma, and other cell-free body fluids using the BioSprint 15 workstation - (EN) Found in: User-Developed Protocols Show details For purification of DNA from stool samples for pathogen detection using the BioSprint 15 DNA Blood Kit - (EN) Found in: User-Developed Protocols Show details Isolation of DNA from soft tissue using the TissueLyser and MagAttract® Mini M48 Kit - (EN) Found in: User-Developed Protocols Show details Isolation of total RNA from paraffin-embedded sections using the MagAttract® RNA Tissue Mini M48 Kit - (EN) Found in: User-Developed Protocols Show details Concentration of RNA in 96-well plates using the MinElute® 96 UF PCR Purification Kit - (EN) Found in: User-Developed Protocols Show details Transient transfection of HEK 293 cells in 75 cm3 flasks using PolyFect® Transfection Reagent - (EN) This procedure has been developed by customers for the transient transfection of HEK 293 cells in 75 cm3 flasks using QIAGEN® PolyFect® Transfection Reagent. It has not been thoroughly tested and optimized by QIAGEN. Found in: User-Developed Protocols Show details (EN) - Rapid extraction of DNA from sperm using the QIAsymphony DNA Investigator Kit This protocol has been adapted from the pretreatment of sexual assault samples and is for the lysis and extraction of DNA from a sperm pellet after differential separation. Found in: User-Developed Protocols Show details Isolation of plasmid DNA from Proteus spp. using the QIAGEN® Plasmid Midi Kit - (EN) The procedure has been used successfully for isolation of high-copy-number plasmids from Proteus vulgaris and Proteus mirabilis. Yield of plasmid DNA was typically 3-8 µg DNA per ml culture. Found in: User-Developed Protocols Show details Isolation of plasmid DNA from Oligotropha carboxidovorans using the QIAGEN® Plasmid Midi Kit - (EN) The procedure has been used successfully for isolation of the large (128 kb), very-low-copynumber (1-2 copies per cell) plasmid pHCG3 and its derivatives from Oligotropha carboxidovorans. Yield of plasmid DNA was typically 3-6 µg plasmid DNA from 200 ml culture. Found in: User-Developed Protocols Show details Isolation of plasmid DNA from Lactobacillus spp. using the QIAGEN® Plasmid Midi Kit - (EN) The procedure has been used successfully for isolation of cryptic plasmids (pLC2-based) from mesophilic Lactobacillus strains such as L. sake and L. curvatus. Yield of plasmid DNA was typically 10-20 µg plasmid DNA from 100 ml culture. Found in: User-Developed Protocols Show details Isolation of very low-copy plasmids from Streptomyces spp. using the QIAGEN® Plasmid Maxi Kit - (EN) The procedure has been used successfully for isolation of plasmids SCP2 and SCP2* as well as the plasmids listed in Table 1 (see next page) from S. coelciolor and S. lividans strains. Found in: User-Developed Protocols Show details Isolation of plasmid DNA from yeast using the QIAGEN® Plasmid Midi Kit - (EN) Found in: User-Developed Protocols Show details Isolation of plasmid DNA from Staphylococcus spp. using the QIAGEN® Plasmid Midi Kit - (EN) The procedure has been used successfully for isolation of a variety of medium-copy-number shuttle vectors from S. xylosus, S. carnosus, S. epidermidis, and S. aureus. Yield of plasmid DNA was typically 2-10 µg from 50 ml culture. Found in: User-Developed Protocols Show details Isolation of bacteriophage-P1-derived constructs using the QIAGEN® Plasmid Midi Kit - (EN) The procedure has been used successfully for isolation of 110 kb P1 DNA (pAdsacBII with an 80 kb insert) from Escherichia coli strain NS3529. Yield of P1 DNA was typically 10-50 µg from 500 ml culture. Found in: User-Developed Protocols Show details Isolation of plasmid DNA from Bacillus subtilis using the QIAGEN® Plasmid Midi Kit - (EN) The procedure has been used successfully for isolation of high- and low-copy-number plasmids from various Bacillus subtilis strains. Yield of plasmid DNA was typically 10-20 µg plasmid DNA from 100 ml culture. Found in: User-Developed Protocols Show details Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care QIAGEN Products & Catalog Sample and Assay Technologies Go to catalog! 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Supplementary Protocols Supplementary Protocols
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Validation Reports Validation Reports
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Supplementary Files Supplementary Files
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Isolation of genomic DNA from flies using the QIAGEN® Genomic-tip - (EN)

Lysis time will vary depending on the size and density of the source material. The QIAGEN Genomic-tip will run slowly due to the high debris content in the nuclear fraction. The yield is about 0.3-0.4 µg DNA/fly.

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