| Cell Line Species/Tissue: |
human
/
embryonic kidney |
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM (high glucose) |
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well |
Number of Cells:
|
|
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
0.6 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
4.5µl |
Complex Incubation on Cells (hrs):
|
40 hrs |
Analysis Performed Post-Transfection (hrs):
|
40 hrs |
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| less plasmid was used |
| Notes: |
| |
| References: |
| |