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The challenge with libraries from single cells or low amounts of RNA is that it can be difficult to detect large numbers of transcripts in your single-cell RNA sequencing applications. To overcome this, we provide an end-to-end library preparation solution for RNA-seq from single cells or low amounts of RNA. The PCR-free, single cell RNA-seq protocol reduces bias and provides exceptional reproducibility and higher diversity libraries for your analyses of the single-cell transcriptome. Plus, you can obtain sequencing-ready libraries from isolated single cells in just 5.5 hours.