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ipsogen Leukemia
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ipsogen Leukemia
ipsogen
RUNX1-RUNX1T1 Kit
ipsogen
RUNX1-RUNX1T1 Kit
采用real-time PCR对RUNX1-RUNX1T1(或AML1-ETO)融合基因转录本进行定量检测
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
ipsogen
RUNX1-RUNX1T1 Kit (24)
Cat no. / ID.
675013
For 24 reactions: ABL Control Gene Standards, RUNX1-RUNX1T1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix RUNX1-RUNX1T1 Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
灵敏的定量检测试剂
对与ABL对照基因表达相关的转录本进行准确定量
符合EAC要求的标准化试剂,结果具有可重复性
卓越的质量保证,获得ISO 13485认证
即用型解决方案,便利的工作流程
产品详情
ipsogen
RUNX1-RUNX1T1 Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。
ipsogen
RUNX1-RUNX1T1 Kit中的试剂经过优化,能够对骨髓或外周血样本中的RUNX1-RUNX1T1(或AML1-ETO)融合基因转录本相对于ABL对照基因的表达的可靠、灵敏的相对定量检测。
绩效
为确保获得高检测灵敏度,
ipsogen
RUNX1-RUNX1T1 Kit经过优化,能够检测宽泛的线性范围内的ABL和RUNX1-RUNX1T1融合基因转录本(参见
Sensitive detection of RUNX1-RUNX1T1
和
ABL standards
)。
原理
ipsogen
RUNX1-RUNX1T1 Kit为即用型试剂盒,采用real-time PCR技术,检测RUNX1-RUNX1T1融合基因转录本。该试剂盒可扩增并检测特定的RUNX1-RUNX1T1融合基因转录本(参见
RUNX1-RUNX1T1 fusion gene transcript
和
ABL control gene transcript
),检测特异性、灵敏度和可重复性高。
ipsogen
RUNX1-RUNX1T1 Kit为ABL基因提供3个标准品稀释液,为RUNX1-RUNX1T1融合基因提供5个标准品稀释液。试剂盒中的引物探针对的设计符合欧洲抗癌协会(Europe Against Cancer,简称EAC)的推荐。
程序
第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。
ipsogen
RUNX1-RUNX1T1 Kit可对RUNX1-RUNX1T1融合基因和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
RUNX1-RUNX1T1 Ki能够对人类细胞中RUNX1-RUNX1T1基因转录本相对于ABL对照基因的表达的可靠、灵敏的定量检测。仅适用于科研。
辅助数据和图表
Sensitive detection of RUNX1-RUNX1T1 and ABL standards.
Detection of [
A
] RUNX1-RUNX1T1 standards (F1 to F5) with 10
1
, 10
2
, 10
3
, 10
5
, and 10
6
copies/5 μl and [
B
] ABL standards (C1, C2, C3) with 10
3
, 10
4
, and 10
5
copies/5 μl.
资源
试剂盒操作手册 (1)
(EN) - ipsogen FusionQuant Handbook
EN
PDF
(937KB)
安全数据表 (1)
Safety Data Sheets
EN
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
EN
Kit Handbooks (1)
(EN) - ipsogen FusionQuant Handbook
EN
PDF
(937KB)
Safety Data Sheets (1)
Safety Data Sheets
EN
Download Safety Data Sheets for QIAGEN product components.